Determination Pantoprazole on Zodiac HST TH Column

Pantoprazole, a proton pump inhibitor (PPI), is commonly prescribed for the treatment of gastroesophageal reflux disease (GERD) and peptic ulcers. The compound can be effectively retained and separated using the Zodiac HST TH mixed-mode stationary phase column. This column enables the separation of analytes with varying ionic characteristics by utilizing both hydrophobic and ion-exchange interactions.

Pantoprazole can be analyzed using an isocratic method with a simple, MS-compatible mobile phase consisting of acetonitrile (ACN), water, and ammonium acetate (AmAc) buffer. Detection can be performed using UV at 290 nm, Evaporative Light Scattering Detector (ELSD), Charged Aerosol Detection (CAD), or Liquid Chromatography-Mass Spectrometry (LC/MS).