Description
HPLC Method for Water-Soluble Vitamins (B1, B2, B3, B6) Analysis
Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, and Vitamin B6 (Pyridoxine, Pyridoxamine, Pyridoxal Phosphate) can be effectively retained, separated, and analyzed using the Zodiac HST OG mixed-mode stationary phase column. This gradient method utilizes a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid buffer, with UV detection at 300 nm.
Condition
Column Name : Zodiac HST OG
Compound Name : Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine,
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