Analysis of Water-Soluble Vitamins (B1, B2, B3, B6) on Zodiac HST OG Column

Home Analysis of Water-Soluble Vitamins (B1, B2, B3, B6) on Zodiac HST OG Column

Description

HPLC Method for Water-Soluble Vitamins (B1, B2, B3, B6) Analysis
Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, and Vitamin B6 (Pyridoxine, Pyridoxamine, Pyridoxal Phosphate) can be effectively retained, separated, and analyzed using the Zodiac HST OG mixed-mode stationary phase column. This gradient method utilizes a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid buffer, with UV detection at 300 nm.

Condition

 

 

  1. Pyridoxal Phosphate
  2. Vitamin B2 (Riboflavin)
  3. Nicotinamide
  4. Vitamin B6 (Pyridoxine)
  5. Pyridoxamine
  6. Vitamin B1 (Thiamine)

Column Name : Zodiac HST OG

Compound Name : 1.Pyridoxal Phosphate

                                  2.Vitamin B2 (Riboflavin)

                                  3.Nicotinamide

                                 4.Vitamin B6 (Pyridoxine)

                                 5.Pyridoxamine

                                6. Vitamin B1 (Thiamine)

              

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