Analysis of Testosterone on Zodiac HST B

Testosterone, the primary male sex hormone, is also present in smaller quantities in females. It plays a vital role in the development of male reproductive organs such as the testes and prostate, while also contributing to secondary sexual characteristics, including increased muscle and bone mass and the growth of body hair. Beyond its reproductive and physical functions, testosterone is essential for overall health and well-being.

The separation, retention, and analysis of testosterone can be effectively achieved using the Zodiac HST B mixed-mode stationary phase column. This method employs a simple isocratic approach with a mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is carried out at UV 270 nm, ensuring accurate and reliable results.