Description
Phosphoserine is a phosphorylated form of the amino acid serine, created when a phosphate group binds to its hydroxyl group. It plays an essential role in cellular signaling and metabolism, serving as a precursor for the synthesis of critical biomolecules like phospholipids, proteins, and nucleotides. Additionally, phosphoserine is integral to protein phosphorylation, a key post-translational modification that controls enzyme activity and signaling pathways.
Phosphoserine can be effectively separated, retained, and analyzed using the Zodiac HST B mixed-mode stationary phase column. The analysis employs a simple isocratic method with a mobile phase consisting of water, acetonitrile (MeCN), and phosphoric acid as a buffer. Detection is performed at UV 200 nm, ensuring precise and reproducible results.
Condition
Column Name : Zodiac HST B
Compound Name : Phosphoserine
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