Analysis of Phenformin on Zodiac HST OG Column

Biguanides such as Moroxydine, Metformin, Proguanil, and Phenformin share a common structural backbone with two guanidine groups.

• Phenformin, once used for type 2 diabetes, was withdrawn in many countries due to the risk of lactic acidosis.

• These biguanides have diverse applications, including antidiabetic, antiviral, and antimalarial treatments, requiring careful medical supervision.

Phenformin can be retained and analyzed using a Zodiac HST OG mixed-mode column with an isocratic method (water, MeCN, ammonium formate buffer) and UV detection at 240 nm.