Analysis of Pefloxacin on Zodiac HST OG

Pefloxacin is an antibiotic from the fluoroquinolone class, primarily used to treat bacterial infections by inhibiting the DNA gyrase enzyme, which is crucial for bacterial DNA replication and repair. Due to this mechanism, Pefloxacin is effective against a range of gram-negative and some gram-positive bacteria.

The retention, separation, and analysis of Pefloxacin can be achieved using a Zodiac HST OG mixed-mode stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer, with detection performed using UV at 200 nm.