Analysis of Neopterin on Zodiac HST OG Column

Biopterin, a pteridine compound, is essential for neurotransmitter synthesis, including serotonin, dopamine, and epinephrine, influencing mood, sleep, and stress response. Metabolic deficiencies can lead to neurological disorders like BH4 deficiency.

Biopterin is analyzed using a Zodiac HST OG column (4.6 × 150 mm, 5 µm, 100 Å) with a mobile phase of water, acetonitrile (MeCN), and ammonium formate. Detection methods include UV at 275 nm, ELSD, CAD, and ESI-MS. The Limit of Detection (LOD) varies depending on instrumentation and laboratory conditions.