Analysis of Kynurenine on Zodiac HST OG Column

Kynurenine is a key metabolite in the tryptophan catabolic pathway, commonly known as the kynurenine pathway. This pathway plays a crucial role in breaking down the essential amino acid tryptophan into various bioactive compounds.

Kynurenine can be retained, separated, and analyzed using a Zodiac HST OG mixed-mode stationary phase column. The analysis follows an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer, with detection performed using UV at 200 nm.