Analysis of Kynurenic Acid on Zodiac HST OG

Kynurenic acid is a metabolite in the kynurenine pathway, which processes the amino acid tryptophan. It acts as an antagonist at several brain receptors, including NMDA and α7-nicotinic acetylcholine receptors, and plays a complex role in the central nervous system. While it has neuroprotective effects—such as reducing excitatory neurotransmission and oxidative stress—elevated levels have been linked to cognitive dysfunction and neurological disorders like schizophrenia and depression.

HPLC Analysis:

The compound can be effectively retained, separated, and analyzed using the Zodiac HST OG mixed-mode column. The method utilizes a gradient elution with a mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is carried out by UV at 240 nm, offering reliable separation and sensitivity.