Analysis of Isomers of Phenylenediamine on Zodiac HST OG Column

Home Analysis of Isomers of Phenylenediamine on Zodiac HST OG Column

Description

Lets explore the capabilities of Zodiac HST OG Column in separation of the positional isomers of ortho, meta , and para Phenylenediamine,

Zodiac HST OG with an innovation chemistry can offers a mixed-mode separation mechanism combining reversed-phase, cation-exchange interactions and Ion exclusion mechanisms

Why this order ?

1. o-Phenylenediamine (ortho) – Least basic (pKa ~4.5)
* The two -NH₂ groups are next to each other.
* They form strong intramolecular hydrogen bonds, which reduces their ability to interact with the stationary phase.
* Less retention → elutes first

2. m-Phenylenediamine (meta) – Intermediate basic (pKa ~5.0)
* The -NH₂ groups are further apart, no intramolecular H-bonding.
* Moderate interaction with stationary phase → moderate retention

3. p-Phenylenediamine (para) – Most basic (pKa ~6.0),
* NH₂ groups are opposite each other → symmetry allows stronger, more stable interaction with stationary phase.
* Both groups are fully available for interaction (especially ion-exchange).
* Strongest retention → elutes last

Condition

Column Name : Zodiac HST OG

Compound Name : 1.o-Phenylenediamine

                                     2.m-Phenylenediamine

                                     3.p-Phenylenediamine

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