Analysis of Glutathione oxidized (GSSG) on Zodiac HST OG Column

Oxidized glutathione (GSSG), also known as glutathione disulfide, is the oxidized form of glutathione (GSH), a key cellular antioxidant. When glutathione neutralizes free radicals or reactive oxygen species (ROS), it forms GSSG. The GSH-to-GSSG ratio serves as a crucial indicator of oxidative stress within cells.Cells regenerate GSH from GSSG using the enzyme glutathione reductase, provided there is sufficient NADPH, which plays a vital role in cellular antioxidant defense mechanisms.

Chromatographic Analysis:

GSSG can be retained and analyzed using a Zodiac HST OG reverse-phase column (4.6 × 150 mm, 5 µm, 100 Å) with a mobile phase of water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method is monitored using UV detection at 200 nm.