Analysis of Cytokinins: A Phytohormone Class and Brassinazole on Zodiac HST-OG2 Column

Home Analysis of Cytokinins: A Phytohormone Class and Brassinazole on Zodiac HST-OG2 Column

Description
  • trans-Zeatin: A naturally occurring cytokinin in plants that promotes cell division and growth.

  • Kinetin: A synthetic cytokinin commonly used in plant tissue culture to stimulate cell division.

  • N6-(Δ2-Isopentenyl)adenine (iP): A plant-derived cytokinin involved in regulating growth, development, and differentiation.

  • N6-Benzyladenine (BA): A synthetic cytokinin widely used in agriculture to enhance plant growth and delay aging.

  • Brassinazole: Not a cytokinin but a brassinosteroid biosynthesis inhibitor, often used to study the role of brassinosteroids in plants.

  • Thidiazuron (TDZ): A phenylurea-derived compound with cytokinin-like properties, frequently used in plant tissue culture for shoot regeneration.

These compounds, including trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, and Brassinazole, can be effectively separated, retained, and analyzed using a Zodiac HST OG2 mixed-mode stationary phase column. The analysis is performed using an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid as a buffer. Detection is carried out at UV wavelengths of 290 nm and 265 nm.

Condition

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