Analysis of Crystal Violet on Zodiac HST OG Column

Crystal Violet (Methyl Violet 10B) is a synthetic triarylmethane dye (C₂₅H₃₀ClN₃) widely used in microbiology, histology, and analytical chemistry. It serves as a Gram stain in bacterial classification, a cell viability stain in biological assays, and an indicator in titrations. Additionally, it has applications in the textile and paper industries.

Chromatographic Analysis:

Crystal Violet can be retained and analyzed on a Zodiac HST OG mixed-mode stationary phase column using an isocratic mobile phase of water, acetonitrile (MeCN), and sulfuric acid (H₂SO₄) as a buffer. Detection occurs in the UV-Vis range at 540, 590, and 200 nm.