Analysis of Amphotericin B on Zodiac HST OG Column

Amphotericin B is a broad-spectrum antifungal from the polyene class, used to treat serious fungal infections.

Mechanism of Action

• Binds to ergosterol in fungal cell membranes, disrupting integrity and causing cell death.

Spectrum of Activity

• Effective against Candida, Aspergillus, Cryptococcus neoformans, and various molds.

Clinical Use

• Used for severe fungal infections but requires careful monitoring due to potential toxicity.

Amphotericin B can be retained and analyzed using a Zodiac HST OG mixed-mode column with an isocratic method employing water, acetonitrile (MeCN), and sulfuric acid as a buffer, with Vis detection at 408 nm.