Analysis of 6-Benzylaminopurine on Zodiac HST OG

6-Benzylaminopurine (6-BAP or BAP) is a synthetic cytokinin widely used in plant biology and tissue culture to promote cell division and growth. It serves as a powerful growth regulator, particularly effective for shoot induction and plant tissue differentiation. As one of the most commonly utilized cytokinins in horticulture and laboratory research, it plays a crucial role in plant development studies.

The retention, separation, and analysis of 6-Benzylaminopurine can be efficiently achieved using a Zodiac HST OG Column with a mixed-mode stationary phase. The analysis follows an isocratic method, utilizing a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is carried out using UV at 210 nm, ensuring accurate and reliable results