Alltesta HPLC Method for Separating Corticosteroids such as Prednisone, Prednisolone and Methylprednisolone on Zodiac HST B Column

All three compounds—Prednisone, Prednisolone, and Methylprednisolone—exhibit strong anti-inflammatory properties, making them essential in managing a wide range of conditions driven by inflammation.

Prednisone

• Mechanism of Action: A synthetic corticosteroid that mimics cortisol, prednisone binds to glucocorticoid receptors and alters gene expression to reduce inflammation and immune response.

• Medical Applications: Commonly prescribed for autoimmune disorders, allergic reactions, inflammatory diseases, and certain types of cancers.

Prednisolone

• Active Form: Prednisolone is the metabolically active form of prednisone, produced in the liver after oral administration of prednisone.

• Pharmacological Action: Similar to prednisone, it provides anti-inflammatory and immunosuppressive effects.

Methylprednisolone

• Potency: Known for its greater potency compared to prednisone.

• Clinical Use: Utilized in more severe cases of inflammation, allergic responses, and as part of immunosuppressive therapies.

These corticosteroids can be retained, separated, and analyzed using the Zodiac HST B mixed-mode stationary phase column. The method employs a simple isocratic mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is carried out using UV at 245 nm, providing reliable results suitable for pharmaceutical analysis.