Alltesta HPLC Method for Analysis of Berberine on Zodiac HST B Column

Berberine is a naturally occurring alkaloid present in various plants, particularly in the roots, rhizomes, and stem bark of species such as Berberis (barberry), Coptis chinensis (goldthread), and Hydrastis canadensis (goldenseal).

Biological Activities

• Medicinal Properties: Traditionally used for its antimicrobial, anti-inflammatory, and antioxidant effects.

• Blood Sugar Regulation: Emerging research indicates that berberine may help regulate blood glucose and enhance insulin sensitivity, making it of significant interest in type 2 diabetes management.

• Cholesterol Control: Studied for its lipid-lowering potential.

• Anticancer Potential: Preclinical studies suggest promising anticancer activities.

Uses

• Dietary Supplements: Berberine is widely available in supplement form for various health applications.

• Traditional Medicine: Utilized in numerous cultures for treating a range of conditions.

Berberine can be effectively retained and analyzed using the Zodiac HST B mixed-mode stationary phase column. The method applies a simple isocratic mobile phase composed of water, acetonitrile (MeCN), and sulfuric acid as the buffer. UV detection at 275 nm ensures accurate and sensitive quantification of the compound.