Dual-Mode HPLC Analysis of Dihydroxybenzoic Acid Isomers

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Description

The Zodiac HST B column provides superior selectivity compared to traditional C18 columns for separating regioisomers of aromatic dihydroxybenzoic acids (e.g., 2,3-, 2,4-, 2,5-, 3,4-, and 3,5-dihydroxybenzoic acids). It utilizes a combination of reversed-phase and anion-exchange mechanisms with a mass spec-compatible mobile phase of water, acetonitrile (MeCN), and trifluoracetic acid (TFA) for efficient separation.

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