ZODIAC HST Method for Separating Corticosteroids such as Prednisone, Prednisolone and Methylprednisolone on Zodiac HST B Column

Prednisone, Prednisolone, and Methylprednisolone are potent corticosteroids with anti-inflammatory and immunosuppressive effects, widely used to manage inflammatory conditions, autoimmune disorders, allergies, and certain cancers.

• Prednisone: A synthetic corticosteroid that regulates gene expression by binding to glucocorticoid receptors.
• Prednisolone: The active metabolite of prednisone, converted in the liver, with similar therapeutic properties.
• Methylprednisolone: A more potent corticosteroid, effective in severe allergies, inflammation, and immunosuppression.

These corticosteroids can be efficiently separated and analyzed using the Zodiac HST B mixed-mode column. The method employs an isocratic mobile phase of water, acetonitrile (MeCN), and sulfuric acid, with UV detection at 245 nm for precise analysis.