Description
Dihydroxybenzoic acids, polar acidic compounds with hydroxyl and carboxylic acid groups, are ideal candidates for separation using the Zodiac HST HB column via hydrogen-bonding mechanisms. Isomers are separated based on the accessibility of their hydroxyl groups. Retention times can be adjusted by varying acetonitrile (ACN), methanol (MeOH), and additive concentrations (formic acid, ammonium formate, triethylamine, etc.). This method is fully compatible with LC/MS and preparative chromatography and can be applied to other hydroxyl- and carboxylic acid-containing compounds.
Condition
Column Name : Zodiac HST HB
Compound Name : 2,6-dihydroxybenzoic acid, 2,4-dihydroxybenzoic acid, 3,5-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid
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