Description
The Zodiac HST OG2 column achieves baseline resolution for nucleic bases (uracil, thymine, cytosine, guanine, and adenine) through a combination of cation exchange and reversed-phase mechanisms. Its embedded anionic functional group enhances retention of polar compounds via polar and ion-exchange interactions. This method delivers excellent peak shapes with a mass-spec-compatible mobile phase of water, acetonitrile (MeCN/ACN), and trifluoroacetic acid (TFA), with UV detection at 270 nm.
Condition
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