HPLC Analysis of Uridine Sugar Diphospates on Zodiac HST- S3 Column

Uridine sugar diphosphates, including UDP-Galactose, UDP-Glucose, and UDP-N-acetylglucosamine (UDP-GlcNAc), are vital nucleotide sugars involved in numerous cellular metabolic processes. These compounds can be detected in the low UV range. Using a ZODIAC HST S3 mixed-mode stationary phase column, these uridine sugar diphosphates can be retained, separated, and analyzed. The analysis employs an isocratic method with a mobile phase comprising water, acetonitrile (MeCN), and an ammonium formate (AmFm) buffer. Detection is achieved using UV at 260 nm, providing accurate separation and identification of these compounds.