HPLC MS Analysis of Alexidine on Zodiac HST- S3 Column

Alexidine is a bis-biguanide antiseptic with broad-spectrum antimicrobial properties, commonly used in healthcare settings for disinfection purposes. It is effective against a wide range of microorganisms, including bacteria, fungi, and some viruses. Alexidine can be retained and analyzed using a ZODIAC HST S3 mixed-mode stationary phase column. The analysis employs an analytical method with a mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using LC-MS, providing accurate and reliable results for the analysis of Alexidine.