Zodiac HST HB

• Hydrogen Bonding Specificity:
  The Zodiac HST HB Column uses a stationary phase engineered to separate compounds primarily through hydrogen bonding interactions. Molecules with polar X-H bonds (e.g., alcohols, amines, acids, phenols, and amides) exhibit higher retention, while hydrocarbons demonstrate minimal retention, ensuring clear separation.
• Non-Aqueous Mobile Phase Compatibility:
  • Operates with acetonitrile (MeCN) and methanol (MeOH) as the mobile phase, optimizing hydrogen bond interactions.
  • MeOH, as a strong solvent, interacts significantly with the stationary phase, modulating analyte retention.
• Wide Application Range:
  Suitable for analyzing a broad spectrum of compounds, including isomers, polar molecules, related impurities, oxidation/reduction products, and drug metabolites.
• Unique Selectivity:
  • Selectivity based on molecular geometry, functional groups, and hydrogen bond interaction points.
  • Efficient separation of structurally similar compounds, such as isomers and homologs.

Advantages :

• Speed:
  • The MeCN/MeOH mobile phase combination has 2–3 times lower viscosity than water-based systems.
  • Enables high-speed separations with smaller particles, achieving UPLC-like performance on standard HPLC instruments.
  • Shortens analysis time up to 5x and further increases speed when using UPLC equipment.
• Solubility:
  • MeOH-MeCN mixtures dissolve a wide range of polar and non-polar molecules, from sugars and salts to lipids and surfactants.
• Enhanced Preparative Chromatography:
  • Low boiling points of MeCN and MeOH facilitate easy solvent evaporation.
  • Mobile phase is MS-friendly, supporting MS-driven sample collection and isocratic methods to minimize solvent consumption.
• Improved Selectivity:
  • Ideal for complex separations of compounds with similar properties.