Zodiac HST B Column
Zodiac HST B Column: Best for Acidic-Hydrophobic and Neutral analytes
The Zodiac HST B column is a high-performance, strong basic mixed-mode analytical column designed to separate a broad range of analytes. Featuring basic ion-pairing groups embedded on long ligand chains, this column excels in providing enhanced retention and selectivity, especially for hydrophobic and acidic components in complex mixtures. With a pKa value of 10, it is well-suited for diverse chromatographic applications.
Why Choose Zodiac HST B Column?
The Zodiac HST B column is ideal for complex separations, offering high resolution, reproducibility, and versatility for applications involving acidic and hydrophobic compounds. Its unique selectivity and compatibility with a variety of mobile phases make it a top choice for high-performance chromatographic workflows.
- Mixed-Mode Separation: Combines reverse-phase and anion-exchange mechanisms to effectively separate acidic analytes, offering enhanced selectivity.
- Eliminates Silanol Interactions: Strong bases, like quaternary amines, often face poor performance due to strong silanol interactions. The Zodiac HST B column, with its positively charged surface, eliminates these interactions, ensuring efficient separation and symmetrical peak shapes.
- Unique Selectivity for Basic Compounds: While basic compounds are retained through reverse-phase interactions, the presence of positively charged groups on both the analyte and stationary phases enables unique selectivity via ion-exclusion phenomena.
- Optimal pH Range: Operates within a pH range of 1.0 to 4.5, using acids like trifluoroacetic, phosphoric, perchloric, or formic acids to control the mobile phase.
- Broad Mobile Phase Compatibility: Offers flexibility with a wide range of organic modifiers (0-100%) to adjust retention and selectivity.
- Mobile Phase Compatibility: Fully compatible with LC-MS, preparative chromatography, and low UV detection applications.
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