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Chromatographic conditions:
Column : Zodiac C18, 150 x 4.6mm, 3µ
Flow Rate : 1.0 mL/min
Column Temp : 25°C
Sample Temp : 25°C
Wavelength : 276 nm
Injection volume : 20 µl
Mobile phase -A: Water : gl. Acetic acid (990:10)
Mobile phase -B : Acetonitrile
Diluent : Water: ACN : g. Acetic acid (500:500:1)
Gradient:
| Time | %A | %B |
| 0 | 90 | 10 |
| 5 | 90 | 10 |
| 32 | 80 | 20 |
| 55 | 50 | 50 |
| 58 | 90 | 10 |
| 65 | 90 | 10 |
Column Name : Zodiac C18 Column
Compound Name : Methocarbamol, Guaifensin isomer, Methocarbamol dioxolon, Guaiacol
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