Home > Product > Zodiac C8- i3 Column
 Zodiac C8 -i3column

Zodiac C8- i3 Column

 

Zodiac C8-i3column, An excellent resolution for critical separations, faster analysis compared to C18 increased selectivity, exceptional reproducibility. Zodiac C8-i3 is an admirable choice for separations that need marginally more polar phase than C18. Zodiac C8-i3 column provides additional resolution to polar phase. Zodiac C8-i3 column is less hydrophobic column with high surface area, optimal carbon load and unique bonded silica packing.

This column is ideal choice when analytical laboratories need extra selectivity and more speed than the C18 column. The superior resolution of Zodiac C8-i3 yields out and out separation first time, speeding up method development and validation. These columns are highly stable and produce ever reliable results with an extended column life. 

The column comes with protective end-capping for a better peak without tailing and high durability of column. Faster, sharp and reproducible responses are definitely possible with Zodiac C8-i3 columns. Column packing sustains any pH range between 1.5 and 8 for longer periods due to the highly innovative bonding technology.

Technical Details

Silica Type

:

 Spherical

Silica Purity

:

 99.999%

Carbon Load

:

 12 %

Surface Area

:

 340 m2/gram

Pore Size

:

 100A

PH Range

:

 2 to 8

Particle Size

:

 5um and 10um 

Product Details for zodiac-c8-i3-column will be updated soon.
General HPLC Column Care And Use Instructions
Column Storage and Column Care
General Guidelines
Do not store HPLC columns in buffers. A buffer may precipitate inside the column, resulting in plugged hits and packing material. A buffer may also encourage bacterial growth, which can plug both the column frit and packing material. This is more likely to occur with mobile phases which have close to or 100% buffer. Bacteria may also affect your analytes, and organic products from the dead bacteria may cause "ghost peaks" in chromatograms. Do not store HPLC columns in solvents that degrade easily -- tetrahydrofuran (THF), triethylamine (TEA), and trifluoroacetic acid (TFA). Unstabilized THF can form peroxides which may degrade the column. TEA and TFA are more likely to become contaminated from the lab environment and should be refrigerated during storage. Contamination may change the chromatography or change the column. Be aware of the quality issues with these mobile phase components when you use them for your chromatography.
 
Term Storage
Reversed-phase columns can also be stored in 100% acetonitrile. If you are using a buffered mobile phase, first flush the column with the mobile phase minus the buffer, then with 100% acetonitrile. In addition, a 100% ACN flush prior to long term storage can help remove contaminants from the column.
 
Regeneration of RP packings
RP- packings are C18, C8, C4, C1, C30, CN or Phenyl stationary phases
Flush the column with 20? column volumes Water
Flush the column with 20? column volumes Acetonitrile
Flush the column with 5? column volumes Isopropanol
Flush the column with 20? column volumes Heptane
Flush the column with 5? column volumes Isopropanol
Flush the column with 20 column volumes Acetonitrile
 
 
Regeneration of NP (Normal Phase) packings
NP-packings are Silica, Diol, Nitro and Amino stationary phases
Flush the column with 20 column volumes Heptane
Flush the column with 5 column volumes Isopropanol
Flush the column with 20 column volumes Acetonitrile
Flush the column with 20 column volumes Water
Flush the column with 20 column volumes Acetonitrile
Flush the column with 5 column volumes Isopropanol
Flush the column with 20 column volumes Heptane
 
Column ID Ideal Flow rate
2 0.2
3 0.5
4 1.0
4.6 1.0
8 3.0
10 5.0
20 20.0
32 48.0
50 120.0
 
Regeneration of Ion-Exchange packings
Anion- and Cation-Exchangers (WCX, SCX, WAX and SAX) are Ion-Exchange Packings
Wash with 20 column volumes with the eluent, double the buffer concentration
Wash with 20 column volumes Water (to elute out salts)
Wash with 20 column volumes Acetonitrile (to elute hydrophilic contaminants)
Wash with 5 column vol. Isopropanol (to elute mildly hydrophobic contaminants)
Wash with 20 column volumes Heptane or Hexane or Methylenchloride (to elute strongly hydrophobic contaminants)
Wash with 5 column volumes Isopropanol
Wash with 20 column volumes Acetonitrile
Wash with 20 column volumes Water
Return the column to its original Ion-Exchange condition

Special Offers

Zodiac C18 Column
Zodiac C18 Column

Buy 5 Analytical Columns and get one column free

Pro Clean™ Screw cap with Seals
Pro Clean™ Screw cap with Seals

Buy 100 Packets HPLC Vial(2ml Screw Vials) and get 5 Packets of 2ml Closures free