Zodiac C8- i3 Column

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Zodiac C8- i3 Column

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    Overview

    Zodiac C8-i3column, An excellent resolution for critical separations, faster analysis compared to C18 increased selectivity, exceptional reproducibility. Zodiac C8-i3 is an admirable choice for separations that need marginally more polar phase than C18. Zodiac C8-i3 column provides additional resolution to polar phase. Zodiac C8-i3 column is less hydrophobic column with high surface area, optimal carbon load and unique bonded silica packing.

    This column is ideal choice when analytical laboratories need extra selectivity and more speed than the C18 column. The superior resolution of Zodiac C8-i3 yields out and out separation first time, speeding up method development and validation. These columns are highly stable and produce ever reliable results with an extended column life.

    The column comes with protective end-capping for a better peak without tailing and high durability of column. Faster, sharp and reproducible responses are definitely possible with Zodiac C8-i3 columns. Column packing sustains any pH range between 1.5 and 8 for longer periods due to the highly innovative bonding technology.

    Technical Details

    Silica Type : Spherical

    Silica Purity : 99.999%

    Carbon Load : 12 %

    Surface Area : 340 m2/gram

    Pore Size : 100A

    PH Range : 2 to 8

    Particle Size : 5um and 10um

    Details

    Details will be updated soon……!

    Order Info

    Analytical Columns

    Part numberPhaseparticle size (µm)length (mm)ID (mm)
    ZLS.C8i3.46.300.0510Zodiac C8-i353004.6
    ZLS.C8i3.40.300.0510Zodiac C8-i353004
    ZLS.C8i3.46.250.0510Zodiac C8-i352504.6
    ZLS.C8i3.40.250.0510Zodiac C8-i352504
    ZLS.C8i3.30.250.0510Zodiac C8-i352503
    ZLS.C8i3.20.250.0510Zodiac C8-i352502
    ZLS.C8i3.10.250.0510Zodiac C8-i352501
    ZLS.C8i3.46.200.0510Zodiac C8-i352004.6
    ZLS.C8i3.40.200.0510Zodiac C8-i352004
    ZLS.C8i3.30.200.0510Zodiac C8-i352003
    ZLS.C8i3.20.200.0510Zodiac C8-i352002
    ZLS.C8i3.10.200.0510Zodiac C8-i352001
    ZLS.C8i3.46.150.0510Zodiac C8-i351504.6
    ZLS.C8i3.40.150.0510Zodiac C8-i351504
    ZLS.C8i3.30.150.0510Zodiac C8-i351503
    ZLS.C8i3.20.150.0510Zodiac C8-i351502
    ZLS.C8i3.10.150.0510Zodiac C8-i351501
    ZLS.C8i3.46.125.0510Zodiac C8-i351254.6
    ZLS.C8i3.40.125.0510Zodiac C8-i351254
    ZLS.C8i3.30.125.0510Zodiac C8-i351253
    ZLS.C8i3.20.125.0510Zodiac C8-i351252
    ZLS.C8i3.10.125.0510Zodiac C8-i351251
    ZLS.C8i3.46.100.0510Zodiac C8-i351004.6
    ZLS.C8i3.40.100.0510Zodiac C8-i351004
    ZLS.C8i3.30.100.0510Zodiac C8-i351003
    ZLS.C8i3.20.100.0510Zodiac C8-i351002
    ZLS.C8i3.10.100.0510Zodiac C8-i351001
    ZLS.C8i3.46.75.0510Zodiac C8-i35754.6
    ZLS.C8i3.40.75.0510Zodiac C8-i35754
    ZLS.C8i3.30.75.0510Zodiac C8-i35753
    ZLS.C8i3.20.75.0510Zodiac C8-i35752
    ZLS.C8i3.10.75.0510Zodiac C8-i35751
    ZLS.C8i3.46.50.0510Zodiac C8-i35504.6
    ZLS.C8i3.40.50.0510Zodiac C8-i35504
    ZLS.C8i3.30.50.0510Zodiac C8-i35503
    ZLS.C8i3.20.50.0510Zodiac C8-i35502
    ZLS.C8i3.10.50.0510Zodiac C8-i35501
    ZLS.C8i3.46.33.0510Zodiac C8-i35334.6
    ZLS.C8i3.40.33.0510Zodiac C8-i35334
    ZLS.C8i3.30.33.0510Zodiac C8-i35333
    ZLS.C8i3.20.33.0510Zodiac C8-i35332
    ZLS.C8i3.10.33.0510Zodiac C8-i35331
    ZLS.C8i3.46.30.0510Zodiac C8-i35304.6
    ZLS.C8i3.40.30.0510Zodiac C8-i35304
    ZLS.C8i3.30.30.0510Zodiac C8-i35303
    ZLS.C8i3.20.30.0510Zodiac C8-i35302
    ZLS.C8i3.10.30.0510Zodiac C8-i35301
    ZLS.C8i3.20.20.0510Zodiac C8-i35202
    ZLS.C8i3.46.300.0310Zodiac C8-i333004.6
    ZLS.C8i3.40.300.0310Zodiac C8-i333004
    ZLS.C8i3.46.250.0310Zodiac C8-i332504.6
    ZLS.C8i3.40.250.0310Zodiac C8-i332504
    ZLS.C8i3.30.250.0310Zodiac C8-i332503
    ZLS.C8i3.20.250.0310Zodiac C8-i332502
    ZLS.C8i3.10.250.0310Zodiac C8-i332501
    ZLS.C8i3.46.200.0310Zodiac C8-i332004.6
    ZLS.C8i3.40.200.0310Zodiac C8-i332004
    ZLS.C8i3.30.200.0310Zodiac C8-i332003
    ZLS.C8i3.20.200.0310Zodiac C8-i332002
    ZLS.C8i3.10.200.0310Zodiac C8-i332001
    ZLS.C8i3.46.150.0310Zodiac C8-i331504.6
    ZLS.C8i3.40.150.0310Zodiac C8-i331504
    ZLS.C8i3.30.150.0310Zodiac C8-i331503
    ZLS.C8i3.20.150.0310Zodiac C8-i331502
    ZLS.C8i3.10.150.0310Zodiac C8-i331501
    ZLS.C8i3.46.125.0310Zodiac C8-i331254.6
    ZLS.C8i3.40.125.0310Zodiac C8-i331254
    ZLS.C8i3.30.125.0310Zodiac C8-i331253
    ZLS.C8i3.20.125.0310Zodiac C8-i331252
    ZLS.C8i3.10.125.0310Zodiac C8-i331251
    ZLS.C8i3.46.100.0310Zodiac C8-i331004.6
    ZLS.C8i3.40.100.0310Zodiac C8-i331004
    ZLS.C8i3.30.100.0310Zodiac C8-i331003
    ZLS.C8i3.20.100.0310Zodiac C8-i331002
    ZLS.C8i3.10.100.0310Zodiac C8-i331001
    ZLS.C8i3.46.75.0310Zodiac C8-i33754.6
    ZLS.C8i3.40.75.0310Zodiac C8-i33754
    ZLS.C8i3.30.75.0310Zodiac C8-i33753
    ZLS.C8i3.20.75.0310Zodiac C8-i33752
    ZLS.C8i3.10.75.0310Zodiac C8-i33751
    ZLS.C8i3.46.50.0310Zodiac C8-i33504.6
    ZLS.C8i3.40.50.0310Zodiac C8-i33504
    ZLS.C8i3.30.50.0310Zodiac C8-i33503
    ZLS.C8i3.20.50.0310Zodiac C8-i33502
    ZLS.C8i3.10.50.0310Zodiac C8-i33501
    ZLS.C8i3.46.33.0310Zodiac C8-i33334.6
    ZLS.C8i3.40.33.0310Zodiac C8-i33334
    ZLS.C8i3.30.33.0310Zodiac C8-i33333
    ZLS.C8i3.20.33.0310Zodiac C8-i33332
    ZLS.C8i3.10.33.0310Zodiac C8-i33331
    ZLS.C8i3.46.30.0310Zodiac C8-i33304.6
    ZLS.C8i3.40.30.0310Zodiac C8-i33304
    ZLS.C8i3.30.30.0310Zodiac C8-i33303
    ZLS.C8i3.20.30.0310Zodiac C8-i33302
    ZLS.C8i3.10.30.0310Zodiac C8-i33301
    ZLS.C8i3.20.20.0310Zodiac C8-i33202
    ZLS.C8i3.46.300.1010Zodiac C8-i3103004.6
    ZLS.C8i3.40.300.1010Zodiac C8-i3103004
    ZLS.C8i3.46.250.1010Zodiac C8-i3102504.6
    ZLS.C8i3.40.250.1010Zodiac C8-i3102504
    ZLS.C8i3.30.250.1010Zodiac C8-i3102503
    ZLS.C8i3.20.250.1010Zodiac C8-i3102502
    ZLS.C8i3.10.250.1010Zodiac C8-i3102501
    ZLS.C8i3.46.200.1010Zodiac C8-i3102004.6
    ZLS.C8i3.40.200.1010Zodiac C8-i3102004
    ZLS.C8i3.30.200.1010Zodiac C8-i3102003
    ZLS.C8i3.20.200.1010Zodiac C8-i3102002
    ZLS.C8i3.10.200.1010Zodiac C8-i3102001
    ZLS.C8i3.46.150.1010Zodiac C8-i3101504.6
    ZLS.C8i3.40.150.1010Zodiac C8-i3101504
    ZLS.C8i3.30.150.1010Zodiac C8-i3101503
    ZLS.C8i3.20.150.1010Zodiac C8-i3101502
    ZLS.C8i3.10.150.1010Zodiac C8-i3101501
    ZLS.C8i3.46.125.1010Zodiac C8-i3101254.6
    ZLS.C8i3.40.125.1010Zodiac C8-i3101254
    ZLS.C8i3.30.125.1010Zodiac C8-i3101253
    ZLS.C8i3.20.125.1010Zodiac C8-i3101252
    ZLS.C8i3.10.125.1010Zodiac C8-i3101251
    ZLS.C8i3.46.100.1010Zodiac C8-i3101004.6
    ZLS.C8i3.40.100.1010Zodiac C8-i3101004
    ZLS.C8i3.30.100.1010Zodiac C8-i3101003
    ZLS.C8i3.20.100.1010Zodiac C8-i3101002
    ZLS.C8i3.10.100.1010Zodiac C8-i3101001
    ZLS.C8i3.46.75.1010Zodiac C8-i310754.6
    ZLS.C8i3.40.75.1010Zodiac C8-i310754
    ZLS.C8i3.30.75.1010Zodiac C8-i310753
    ZLS.C8i3.20.75.1010Zodiac C8-i310752
    ZLS.C8i3.10.75.1010Zodiac C8-i310751
    ZLS.C8i3.46.50.1010Zodiac C8-i310504.6
    ZLS.C8i3.40.50.1010Zodiac C8-i310504
    ZLS.C8i3.30.50.1010Zodiac C8-i310503
    ZLS.C8i3.20.50.1010Zodiac C8-i310502
    ZLS.C8i3.10.50.1010Zodiac C8-i310501
    ZLS.C8i3.46.33.1010Zodiac C8-i310334.6
    ZLS.C8i3.40.33.1010Zodiac C8-i310334
    ZLS.C8i3.30.33.1010Zodiac C8-i310333
    ZLS.C8i3.20.33.1010Zodiac C8-i310332
    ZLS.C8i3.10.33.1010Zodiac C8-i310331
    ZLS.C8i3.46.30.1010Zodiac C8-i310304.6
    ZLS.C8i3.40.30.1010Zodiac C8-i310304
    ZLS.C8i3.30.30.1010Zodiac C8-i310303
    ZLS.C8i3.20.30.1010Zodiac C8-i310302
    ZLS.C8i3.10.30.1010Zodiac C8-i310301
    ZLS.C8i3.20.20.1010Zodiac C8-i310202

    Zodiac Preparative Columns

    Part numberPhaseparticle size (µm)length (mm)ID (mm)
    ZLS.C8i3P.100.50.1010Zodiac C8-i3 Prep105010
    ZLS.C8i3P.200.50.1010Zodiac C8-i3 Prep105020
    ZLS.C8i3P.300.50.1010Zodiac C8-i3 Prep105030
    ZLS.C8i3P.400.50.1010Zodiac C8-i3 Prep105040
    ZLS.C8i3P.500.50.1010Zodiac C8-i3 Prep105050
    ZLS.C8i3P.100.100.1010Zodiac C8-i3 Prep1010010
    ZLS.C8i3P.200.100.1010Zodiac C8-i3 Prep1010020
    ZLS.C8i3P.300.100.1010Zodiac C8-i3 Prep1010030
    ZLS.C8i3P.400.100.1010Zodiac C8-i3 Prep1010040
    ZLS.C8i3P.500.100.1010Zodiac C8-i3 Prep1010050
    ZLS.C8i3P.100.150.1010Zodiac C8-i3 Prep1015010
    ZLS.C8i3P.200.150.1010Zodiac C8-i3 Prep1015020
    ZLS.C8i3P.300.150.1010Zodiac C8-i3 Prep1015030
    ZLS.C8i3P.400.150.1010Zodiac C8-i3 Prep1015040
    ZLS.C8i3P.500.150.1010Zodiac C8-i3 Prep1015050
    ZLS.C8i3P.100.200.1010Zodiac C8-i3 Prep1020010
    ZLS.C8i3P.200.200.1010Zodiac C8-i3 Prep1020020
    ZLS.C8i3P.300.200.1010Zodiac C8-i3 Prep1020030
    ZLS.C8i3P.400.200.1010Zodiac C8-i3 Prep1020040
    ZLS.C8i3P.500.200.1010Zodiac C8-i3 Prep1020050
    ZLS.C8i3P.100.250.1010Zodiac C8-i3 Prep1025010
    ZLS.C8i3P.200.250.1010Zodiac C8-i3 Prep1025020
    ZLS.C8i3P.300.250.1010Zodiac C8-i3 Prep1025030
    ZLS.C8i3P.400.250.1010Zodiac C8-i3 Prep1025040
    ZLS.C8i3P.500.250.1010Zodiac C8-i3 Prep1025050
    ZLS.C8i3P.100.300.1010Zodiac C8-i3 Prep1030010
    ZLS.C8i3P.200.300.1010Zodiac C8-i3 Prep1030020
    ZLS.C8i3P.300.300.1010Zodiac C8-i3 Prep1030030
    ZLS.C8i3P.400.300.1010Zodiac C8-i3 Prep1030040
    ZLS.C8i3P.500.300.1010Zodiac C8-i3 Prep1030050
    ZLS.C8i3P.100.500.1010Zodiac C8-i3 Prep1050010
    ZLS.C8i3P.200.500.1010Zodiac C8-i3 Prep1050020
    ZLS.C8i3P.300.500.1010Zodiac C8-i3 Prep1050030
    ZLS.C8i3P.400.500.1010Zodiac C8-i3 Prep1050040
    ZLS.C8i3P.500.500.1010Zodiac C8-i3 Prep1050050
    ZLS.C8i3P.100.50.1510Zodiac C8-i3 Prep155010
    ZLS.C8i3P.200.50.1510Zodiac C8-i3 Prep155020
    ZLS.C8i3P.300.50.1510Zodiac C8-i3 Prep155030
    ZLS.C8i3P.400.50.1510Zodiac C8-i3 Prep155040
    ZLS.C8i3P.500.50.1510Zodiac C8-i3 Prep155050
    ZLS.C8i3P.100.100.1510Zodiac C8-i3 Prep1510010
    ZLS.C8i3P.200.100.1510Zodiac C8-i3 Prep1510020
    ZLS.C8i3P.300.100.1510Zodiac C8-i3 Prep1510030
    ZLS.C8i3P.400.100.1510Zodiac C8-i3 Prep1510040
    ZLS.C8i3P.500.100.1510Zodiac C8-i3 Prep1510050
    ZLS.C8i3P.100.150.1510Zodiac C8-i3 Prep1515010
    ZLS.C8i3P.200.150.1510Zodiac C8-i3 Prep1515020
    ZLS.C8i3P.300.150.1510Zodiac C8-i3 Prep1515030
    ZLS.C8i3P.400.150.1510Zodiac C8-i3 Prep1515040
    ZLS.C8i3P.500.150.1510Zodiac C8-i3 Prep1515050
    ZLS.C8i3P.100.200.1510Zodiac C8-i3 Prep1520010
    ZLS.C8i3P.200.200.1510Zodiac C8-i3 Prep1520020
    ZLS.C8i3P.300.200.1510Zodiac C8-i3 Prep1520030
    ZLS.C8i3P.400.200.1510Zodiac C8-i3 Prep1520040
    ZLS.C8i3P.500.200.1510Zodiac C8-i3 Prep1520050
    ZLS.C8i3P.100.250.1510Zodiac C8-i3 Prep1525010
    ZLS.C8i3P.200.250.1510Zodiac C8-i3 Prep1525020
    ZLS.C8i3P.300.250.1510Zodiac C8-i3 Prep1525030
    ZLS.C8i3P.400.250.1510Zodiac C8-i3 Prep1525040
    ZLS.C8i3P.500.250.1510Zodiac C8-i3 Prep1525050
    ZLS.C8i3P.100.300.1510Zodiac C8-i3 Prep1530010
    ZLS.C8i3P.200.300.1510Zodiac C8-i3 Prep1530020
    ZLS.C8i3P.300.300.1510Zodiac C8-i3 Prep1530030
    ZLS.C8i3P.400.300.1510Zodiac C8-i3 Prep1530040
    ZLS.C8i3P.500.300.1510Zodiac C8-i3 Prep1530050
    ZLS.C8i3P.100.500.1510Zodiac C8-i3 Prep1550010
    ZLS.C8i3P.200.500.1510Zodiac C8-i3 Prep1550020
    ZLS.C8i3P.300.500.1510Zodiac C8-i3 Prep1550030
    ZLS.C8i3P.400.500.1510Zodiac C8-i3 Prep1550040
    ZLS.C8i3P.500.500.1510Zodiac C8-i3 Prep1550050
    ZLS.C8i3P.100.50.2010Zodiac C8-i3 Prep205010
    ZLS.C8i3P.200.50.2010Zodiac C8-i3 Prep205020
    ZLS.C8i3P.300.50.2010Zodiac C8-i3 Prep205030
    ZLS.C8i3P.400.50.2010Zodiac C8-i3 Prep205040
    ZLS.C8i3P.500.50.2010Zodiac C8-i3 Prep205050
    ZLS.C8i3P.100.100.2010Zodiac C8-i3 Prep2010010
    ZLS.C8i3P.200.100.2010Zodiac C8-i3 Prep2010020
    ZLS.C8i3P.300.100.2010Zodiac C8-i3 Prep2010030
    ZLS.C8i3P.400.100.2010Zodiac C8-i3 Prep2010040
    ZLS.C8i3P.500.100.2010Zodiac C8-i3 Prep2010050
    ZLS.C8i3P.100.150.2010Zodiac C8-i3 Prep2015010
    ZLS.C8i3P.200.150.2010Zodiac C8-i3 Prep2015020
    ZLS.C8i3P.300.150.2010Zodiac C8-i3 Prep2015030
    ZLS.C8i3P.400.150.2010Zodiac C8-i3 Prep2015040
    ZLS.C8i3P.500.150.2010Zodiac C8-i3 Prep2015050
    ZLS.C8i3P.100.200.2010Zodiac C8-i3 Prep2020010
    ZLS.C8i3P.200.200.2010Zodiac C8-i3 Prep2020020
    ZLS.C8i3P.300.200.2010Zodiac C8-i3 Prep2020030
    ZLS.C8i3P.400.200.2010Zodiac C8-i3 Prep2020040
    ZLS.C8i3P.500.200.2010Zodiac C8-i3 Prep2020050
    ZLS.C8i3P.100.250.2010Zodiac C8-i3 Prep2025010
    ZLS.C8i3P.200.250.2010Zodiac C8-i3 Prep2025020
    ZLS.C8i3P.300.250.2010Zodiac C8-i3 Prep2025030
    ZLS.C8i3P.400.250.2010Zodiac C8-i3 Prep2025040
    ZLS.C8i3P.500.250.2010Zodiac C8-i3 Prep2025050
    ZLS.C8i3P.100.300.2010Zodiac C8-i3 Prep2030010
    ZLS.C8i3P.200.300.2010Zodiac C8-i3 Prep2030020
    ZLS.C8i3P.300.300.2010Zodiac C8-i3 Prep2030030
    ZLS.C8i3P.400.300.2010Zodiac C8-i3 Prep2030040
    ZLS.C8i3P.500.300.2010Zodiac C8-i3 Prep2030050
    ZLS.C8i3P.100.500.2010Zodiac C8-i3 Prep2050010
    ZLS.C8i3P.200.500.2010Zodiac C8-i3 Prep2050020
    ZLS.C8i3P.300.500.2010Zodiac C8-i3 Prep2050030
    ZLS.C8i3P.400.500.2010Zodiac C8-i3 Prep2050040
    ZLS.C8i3P.500.500.2010Zodiac C8-i3 Prep2050050
    ZLS.C8i3P.100.50.3010Zodiac C8-i3 Prep305010
    ZLS.C8i3P.200.50.3010Zodiac C8-i3 Prep305020
    ZLS.C8i3P.300.50.3010Zodiac C8-i3 Prep305030
    ZLS.C8i3P.400.50.3010Zodiac C8-i3 Prep305040
    ZLS.C8i3P.500.50.3010Zodiac C8-i3 Prep305050
    ZLS.C8i3P.100.100.3010Zodiac C8-i3 Prep3010010
    ZLS.C8i3P.200.100.3010Zodiac C8-i3 Prep3010020
    ZLS.C8i3P.300.100.3010Zodiac C8-i3 Prep3010030
    ZLS.C8i3P.400.100.3010Zodiac C8-i3 Prep3010040
    ZLS.C8i3P.500.100.3010Zodiac C8-i3 Prep3010050
    ZLS.C8i3P.100.150.3010Zodiac C8-i3 Prep3015010
    ZLS.C8i3P.200.150.3010Zodiac C8-i3 Prep3015020
    ZLS.C8i3P.300.150.3010Zodiac C8-i3 Prep3015030
    ZLS.C8i3P.400.150.3010Zodiac C8-i3 Prep3015040
    ZLS.C8i3P.500.150.3010Zodiac C8-i3 Prep3015050
    ZLS.C8i3P.100.200.3010Zodiac C8-i3 Prep3020010
    ZLS.C8i3P.200.200.3010Zodiac C8-i3 Prep3020020
    ZLS.C8i3P.300.200.3010Zodiac C8-i3 Prep3020030
    ZLS.C8i3P.400.200.3010Zodiac C8-i3 Prep3020040
    ZLS.C8i3P.500.200.3010Zodiac C8-i3 Prep3020050
    ZLS.C8i3P.100.250.3010Zodiac C8-i3 Prep3025010
    ZLS.C8i3P.200.250.3010Zodiac C8-i3 Prep3025020
    ZLS.C8i3P.300.250.3010Zodiac C8-i3 Prep3025030
    ZLS.C8i3P.400.250.3010Zodiac C8-i3 Prep3025040
    ZLS.C8i3P.500.250.3010Zodiac C8-i3 Prep3025050
    ZLS.C8i3P.100.300.3010Zodiac C8-i3 Prep3030010
    ZLS.C8i3P.200.300.3010Zodiac C8-i3 Prep3030020
    ZLS.C8i3P.300.300.3010Zodiac C8-i3 Prep3030030
    ZLS.C8i3P.400.300.3010Zodiac C8-i3 Prep3030040
    ZLS.C8i3P.500.300.3010Zodiac C8-i3 Prep3030050
    ZLS.C8i3P.100.500.3010Zodiac C8-i3 Prep3050010
    ZLS.C8i3P.200.500.3010Zodiac C8-i3 Prep3050020
    ZLS.C8i3P.300.500.3010Zodiac C8-i3 Prep3050030
    ZLS.C8i3P.400.500.3010Zodiac C8-i3 Prep3050040
    ZLS.C8i3P.500.500.3010Zodiac C8-i3 Prep3050050

    Zodiac Guard Columns

    Part numberPhaseparticle size (µm)length (mm)ID (mm)
    ZLS.C8i3.40.10.5GZodiac C8-i35104
    ZLS.C8i3.46.30.5GZodiac C8-i35304.6
    ZLS.C8i3.40.30.5GZodiac C8-i35304
    ZLS.C8i3.46.33.5GZodiac C8-i35334.6
    ZLS.C8i3.40.33.5GZodiac C8-i35334
    ZLS.C8i3.46.10.3GZodiac C8-i33104.6
    ZLS.C8i3.40.10.3GZodiac C8-i33104
    ZLS.C8i3.46.30.3GZodiac C8-i33304.6
    ZLS.C8i3.40.30.3GZodiac C8-i33304
    ZLS.C8i3.46.33.3GZodiac C8-i33334.6
    ZLS.C8i3.40.33.3GZodiac C8-i33334

    Support

    General HPLC Column Care And Use Instructions
    Column Storage and Column Care
    General Guidelines
    Do not store HPLC columns in buffers. A buffer may precipitate inside the column, resulting in plugged hits and packing material. A buffer may also encourage bacterial growth, which can plug both the column frit and packing material. This is more likely to occur with mobile phases which have close to or 100% buffer. Bacteria may also affect your analytes, and organic products from the dead bacteria may cause “ghost peaks” in chromatograms. Do not store HPLC columns in solvents that degrade easily — tetrahydrofuran (THF), triethylamine (TEA), and trifluoroacetic acid (TFA). Unstabilized THF can form peroxides which may degrade the column. TEA and TFA are more likely to become contaminated from the lab environment and should be refrigerated during storage. Contamination may change the chromatography or change the column. Be aware of the quality issues with these mobile phase components when you use them for your chromatography.

    Term Storage

    Reversed-phase columns can also be stored in 100% acetonitrile. If you are using a buffered mobile phase, first flush the column with the mobile phase minus the buffer, then with 100% acetonitrile. In addition, a 100% ACN flush prior to long term storage can help remove contaminants from the column.

    Regeneration of RP packings

    RP- packings are C18, C8, C4, C1, C30, CN or Phenyl stationary phases
    • Flush the column with 20? column volumes Water
    • Flush the column with 20? column volumes Acetonitrile
    • Flush the column with 5? column volumes Isopropanol
    • Flush the column with 20? column volumes Heptane
    • Flush the column with 5? column volumes Isopropanol
    • Flush the column with 20 column volumes Acetonitrile

    Regeneration of NP (Normal Phase) packings
    NP-packings are Silica, Diol, Nitro and Amino stationary phases
    •Flush the column with 20 column volumes Heptane
    •Flush the column with 5 column volumes Isopropanol
    •Flush the column with 20 column volumes Acetonitrile
    •Flush the column with 20 column volumes Water
    •Flush the column with 20 column volumes Acetonitrile
    •Flush the column with 5 column volumes Isopropanol
    •Flush the column with 20 column volumes Heptane
    Column IDIdeal Flow rate
    20.2
    30.5
    41.0
    4.61.0
    83.0
    105.0
    2020.0
    3248.0
    50120.0

    Regeneration of Ion-Exchange packings

    Anion- and Cation-Exchangers (WCX, SCX, WAX and SAX) are Ion-Exchange Packings
    • Wash with 20 column volumes with the eluent, double the buffer concentration
    • Wash with 20 column volumes Water (to elute out salts)
    • Wash with 20 column volumes Acetonitrile (to elute hydrophilic contaminants)
    • Wash with 5 column vol. Isopropanol (to elute mildly hydrophobic contaminants)
    • Wash with 20 column volumes Heptane or Hexane or Methylenchloride (to elute strongly hydrophobic contaminants)
    • Wash with 5 column volumes Isopropanol
    • Wash with 20 column volumes Acetonitrile
    • Wash with 20 column volumes Water
    • Return the column to its original Ion-Exchange condition

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