Zodiac 120 RP Amide

Zodiac 120 RP Amide Column

The Zodiac 120 RP Amide column is a reversed-phase HPLC column with an amide-based stationary phase, ideal for separating both polar and non-polar analytes. It offers high resolution and reproducibility in aqueous-organic mobile phases for various analytical applications.

What is an RP Amide Column?

An RP Amide column is a type of reversed-phase HPLC column that incorporates an amide functional group in the stationary phase. Unlike traditional C18 or C8 columns that rely solely on hydrophobic interactions, RP Amide columns utilize a combination of hydrophobic and hydrogen bonding interactions. This dual mechanism provides improved retention and selectivity for polar analytes while maintaining compatibility with aqueous mobile phases.

Key Features of RP Amide Columns

1. Hydrogen Bonding Capability – The presence of the amide functional group enhances hydrogen bonding interactions, allowing for better retention of polar compounds.
2. Improved Aqueous Compatibility – RP Amide columns can operate effectively in high aqueous conditions, reducing peak tailing and improving reproducibility.
3. Alternative Selectivity – Compared to traditional C18 columns, RP Amide columns offer unique selectivity, making them suitable for compounds that do not separate well on standard reversed-phase columns.
4. Enhanced Peak Shape – The amide group minimizes secondary interactions with basic analytes, leading to sharper peaks and more precise quantification.

Advantages of Using RP Amide Columns

• Better Retention of Polar Compounds – Many reversed-phase columns struggle with polar compounds, but RP Amide columns provide enhanced retention due to their dual interaction mechanism.
• Reduced Need for Ion Pairing Agents – Since RP Amide columns can retain polar compounds without requiring ion-pairing reagents, they simplify method development and reduce potential contaminants.
• Higher Sensitivity in LC-MS Applications – The reduced need for ion-pairing agents and compatibility with aqueous mobile phases make RP Amide columns ideal for mass spectrometry applications.
• Improved Peak Symmetry – These columns reduce peak tailing, especially for basic compounds, leading to better resolution and reproducibility.

Application Name

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