Description
This HPLC method uses a Zodiac 120 C18 AQ column with a 30:70 acetonitrile mobile phase to separate and identify Tramadol HCl and its impurities (Impurity-D, RC-A, N-Oxide, Impurity-C, Impurity-B) at 270 nm. The analysis is performed at a flow rate of 1.0 ml/min, with a 20-minute run time and 20 µL injection volume.
Condition
Column Name : Zodiac 120 C18 AQ HPLC Column
Compound Name :
- Impurity-D
- RC-A
- Tramadol Hcl
- N-Oxide
- Impurity-C
- Impurity-B
- Impurity-D
- RC-A
- Tramadol Hcl
- N-Oxide
- Impurity-C
- Impurity-B
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