Description
This HPLC method uses a Zodiac C18/Zodiac C18(1)/Zodiac 120 C18 AQ column with a 30:70 acetonitrile:TFA mobile phase to separate and identify Tramadol HCl and its impurities (Impurity-D, RC-A, N-Oxide, Impurity-C, Impurity-B) at 270 nm. The analysis is performed at a flow rate of 1.0 ml/min, with a 20-minute run time and 20 µL injection volume.
Condition
Column Name : Zodiac C18(1) Column , Zodiac 120 C18 AQ HPLC Column , Zodiac C18 Column
Compound Name : Impurity-D ,RC-A ,Tramadol Hcl ,N-Oxide ,Impurity-C ,Impurity-B
1)Impurity-D
2)RC-A
3)Tramadol Hcl
4)N-Oxide
5)Impurity-C
6)Impurity-B
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Column Name : Zodiac C18(1) Column , Zodiac 120 C18 AQ HPLC Column , Zodiac C18 Column
Compound Name : Impurity-D ,RC-A ,Tramadol Hcl ,N-Oxide ,Impurity-C ,Impurity-B
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1)Impurity-D
2)RC-A
3)Tramadol Hcl
4)N-Oxide
5)Impurity-C
6)Impurity-B
Get Your Quote or Call: 040-29881474
We focus on supporting laboratory workflows & optimizing lab-wide operations