Tramadol Hydrochloride on Zodiac C18

Description

This HPLC method uses a Zodiac C18  column with a 30:70 acetonitrile mobile phase to separate and identify Tramadol HCl and its impurities (Impurity-D, RC-A, N-Oxide, Impurity-C, Impurity-B) at 270 nm. The analysis is performed at a flow rate of 1.0 ml/min, with a 20-minute run time and 20 µL injection volume.

Condition

Column Name  :  Zodiac C18 Column

Compound Name :

1. Impurity-D
2. RC-A
3. Tramadol Hcl
4. N-Oxide
5. Impurity-C
6. Impurity-B

1)Impurity-D
2)RC-A
3)Tramadol Hcl
4)N-Oxide
5)Impurity-C
6)Impurity-B