To separate related substances of Fluorouracil using a Zodiac Phenyl column in high-performance liquid chromatography (HPLC) as outlined in the USP method. The USP method for Fluorouracil typically employs a mobile phase that might include a combination of water, acetonitrile, and a buffer. Prepare a standard solution of Fluorouracil with a known concentration. This solution will be used to create a calibration curve and to identify peaks. Equilibrate the Zodiac Phenyl column with the mobile phase before use. Ensure the system is stabilized.
Condition
Description
| Column Name | Zodiac-Phenyl Column |
| Compound Name | 1)Unknown Imp.1 2)Fluorouracil Related Compound A 3)Unknown Imp.2 4)Fluorouracil Related Compound B 5)Uracil 6)Fluorouracil 7)Unknown Imp.3 8)Fluorouracil Related Compound E |
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