Oligonucleotides

Larger oligonucleotides, their analogs, and dsDNA fragments are analyzed and purified by anion-exchange chromatography. Our PolyWAX LP™ material affords excellent results in such applications, especially the 3-µm, 1500-Å version. G-rich nucleic acids cause problems with some separation methods but not with PolyWAX LP™ columns eluted with a NaClO4 gradient.
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PCR Reaction Products : Results from Raquel Hernandez’ group (North Carolina St. Univ.) demonstrate that PCR reaction products can be purified on a column of this material much more conveniently than with a PAGE gel and with higher recovery [BELOW]. This is true even of GC-rich products.

Application Name : HPLC Application Of oligonucleotides

Column Name : PolyWAX LP™ Columns

Analytes : Oligonucleotides

The 3-µm version of PolyWAX LP™ affords unusually good resolution of oligonucleotides.

Application Name : HPLC Application Of Anion Exchange Of Oligonucleotides

Column Name : PolyWAX LP™ Columns

Analytes : Oligonucleotides