Methocarbomol injection –RS Method

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Chromatographic conditions:

Column : Zodiac C18, 150 x 4.6mm, 3µ

Flow Rate : 1.0 mL/min

Column Temp : 25°C

Sample Temp : 25°C

Wavelength : 276 nm

Injection volume : 20 µl

Mobile phase -A: Water : gl. Acetic acid (990:10)

Mobile phase -B : Acetonitrile

Diluent : Water: ACN : g. Acetic acid (500:500:1)

Gradient:
Time %A %B
0 90 10
5 90 10
32 80 20
55 50 50
58 90 10
65 90 10

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