HPLC Separation of Analgesics

Mobile Phase: A: H2O + 0.1% HCOOH, B: CH3CN + 0.1% HCOOH,
Gradient: 25% – 80% B in 20 mins
Flow: 1.0 ml/min
Detection: UV @ 254 nm

Column Name : Zodiac Phenyl

Compound Name : Ibuprofen, Acetylsalicylic acid (Aspirin), Carbamazepine, Naproxen, Diclofenac, Salicylic acid, Tolmetin, Acetaminophen, Acetanilide, Phenacetin

Column : Zodiac C18
Size : 150mm x 4.6mm, 5um
Mobile Phase : A: H2O+0.1% HCOOH, B: CH3CN+0.1% HCOOH
A:B – 60:40
Flow : 1.0 ml/min
Detection : UV@254 nm

Column Name : Zodiac C18 Column

Compound Name: Acetylsalicylic acid (Aspirin), Naproxen, Homovanillic acid, Sorbic acid, Salicylic acid, o-Toluic acid, Tolmetin