Methods, application insights, and new column updates
Zodiac HST offers advanced high-stability HPLC columns featuring FlexiPhase® technology for complex separations, delivering exceptional selectivity, reproducibility, and reliable performance across challenging analytical applications.


The Zodiac HST A column features strong acidic ion-bearing groups embedded in long ligand chains. It excels in the separation of weakly basic compounds and neutral analytes by leveraging both cation-exchange and reverse-phase mechanisms, making it ideal for a wide range of chromatographic applications.

The Zodiac HST OG column sets the standard for advanced mixed-mode chromatography, offering unparalleled versatility for complex separations. With a low pKa value of 1 and an innovative integration of acidic ion-pairing groups and hydrophobic ligand chains, this column is a game-changer for tackling challenging analytes
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The Zodiac HST OG2 column is designed for the separation of strong bases, dibases, and polar molecules. Featuring a pKa value of 2, it offers excellent retention and reproducibility for complex separations, combining multiple modes of interaction for diverse analytes.
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The Zodiac HST C column is engineered for the separation of amines, metal ions, and neutral compounds. It features a unique combination of cation-exchange and reverse-phase mechanisms, with a focus on host-guest complexation for enhanced retention of amines.
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The Zodiac HST B column is a high-performance strong basic analytical column designed with basic ion-bearing groups (pKa 10) for the effective separation of weak bases, amino acids, and metals.
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The Zodiac HST B2 column is a weak basic high-performance mixed-mode analytical column, uniquely designed to handle a wide range of analytes. Featuring embedded basic group functionality with a pKb of approximately 4.5 and acidic carboxylic groups, this dual-mode column excels in separating complex mixtures

The Zodiac HST-SB column is a high-performance reverse-phase analytical column, meticulously designed for versatile and reliable separations. Featuring robust embedded basic ion-pairing groups and a C18 carbon chain, this column ensures superior retention and resolution for acidic, basic, and neutral analytes.

The Zodiac HST S1 column is a high-performance HILIC analytical column featuring embedded acidic ion-pairing groups. Designed for exceptional versatility, it efficiently separates common sugars and highly polar molecules, making it ideal for challenging analyses.

Zodiac S2 is a silica-based, acidic column with hydrophilic properties. It separates basic compounds using a cation-exchange mechanism, and separates basic and neutral polar compounds through a HILIC (Hydrophilic Interaction Liquid Chromatography) mechanism.

Zodiac HST S3 is a normal-phase analytical column with embedded acidic groups featuring a pKa of about 5. It enhances the retention of basic compounds by cation-exchange mechanism at pH > 5, and separates polar compounds using the HILIC mechanism.

This column is optimized for normal-phase separations and HILIC applications. Unlike conventional HILIC columns, the Zodiac HST N features a unique surface chemistry with both positively and negatively charged functional groups, optimally spaced via a hydrophilic linker, enabling uninterrupted electrostatic interactions.

The Zodiac HST TH column features cationic groups near the silica surface, separated from anionic groups at the tail by a hydrophobic chain. This design allows the interaction of Polar and Ionic analytes at both ends, with Hydrophobic compounds interaction at the ligand chain ensuring high-resolution separations across diverse sample types.

The Zodiac HST P1 column combines reverse-phase, pi-pi, and strong cation-exchange interactions, offering unparalleled versatility. It features negative acid-bearing groups with strong cation-exchange properties, making it ideal for retaining and separating basic analytes and structural isomers of aromatic compounds.

The Zodiac HST P2 column leverages multiple interaction mechanisms including reverse-phase, pi-pi, and strong anion-exchange. It incorporates positive basic-bearing groups with strong anion-exchange capabilities, offering superior separation of acidic analytes and structural isomers of aromatic compounds.

The Zodiac HST RP column redefines plasma and biofluid analysis by eliminating the need for complex, multi-step sample preparation. Engineered with advanced mixed-mode functionality, it features basic ion-pairing groups embedded within long ligand chains, enabling direct injection of plasma and biofluids without compromising column performance or accuracy.
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The Zodiac HST HB Column introduces cutting-edge hydrogen-bonding (H-bonding) chromatography, setting a new standard in separation technology. This innovative column is designed to separate molecules based on their capacity to form hydrogen bonds, leveraging a unique stationary phase for unparalleled selectivity and efficiency among polar compounds.
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Zodiac HST® combines multiple interaction mechanisms—including reversed-phase, ion-exchange, HILIC, π-π interactions, and hydrogen bonding—within a single stationary phase. This FlexiPhase® technology enables analysts to solve difficult separations while simplifying method development and reducing analysis time.
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