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Tramadol Hydrochloride on Zodiac C18(1)
Application ID :
AP_1145
Analytes
- Impurity-D, RC-A, Tramadol Hcl, N-Oxide, Impurity-C, Impurity-B
Conditions
Column : Zodiac C18(1) Column
Column Size : 250mmx4.6mm, 5µm
Mobile Phase :
Solution A: Dissolve 2ml trifluoroacetic acid in 1000ml H2O<br> M.P.: Acetonitrile & Solution A (30:70)
Flow Rate : 1.0 ml/min
Buffer Details : This HPLC method uses a Zodiac C18(1) column with a 30:70 acetonitrile mobile phase to separate and identify Tramadol HCl and its impurities (Impurity-D, RC-A, N-Oxide, Impurity-C, Impurity-B) at 270 nm. The analysis is performed at a flow rate of 1.0 ml/min, with a 20-minute run time and 20 µL injection volume.
Detector : UV 270 nm
Column Temperature : 25°C
Injection Volume : 20ul
Diluent/Sample Solvent : Methanol: Mobile Phase
Description
This HPLC method uses a Zodiac C18(1) column with a 30:70 acetonitrile mobile phase to separate and identify Tramadol HCl and its impurities (Impurity-D, RC-A, N-Oxide, Impurity-C, Impurity-B) at 270 nm. The analysis is performed at a flow rate of 1.0 ml/min, with a 20-minute run time and 20 µL injection volume.
