Methods, application insights, and new column updates
Tramadol Hydrochloride on Zodiac 120 C18 AQ
Application ID :
57
Analytes
- Impurity-D
- RC-A
- Tramadol Hcl
- N-Oxide
- Impurity-C
- Impurity-B
Conditions
Column : Zodiac 120 C18 AQ HPLC Column
Column Size : 250mmx4.6mm, 5µm
Mobile Phase :
Solution A: Dissolve 2ml trifluoroacetic acid in 1000ml
H20<br>
M.P.: Acetonitrile & Solution A (30:70)
Flow Rate : 1.0 ml/min
Description
This HPLC method uses a Zodiac 120 C18 AQ column with a 30:70 acetonitrile mobile phase to separate and identify Tramadol HCl and its impurities (Impurity-D, RC-A, N-Oxide, Impurity-C, Impurity-B) at 270 nm. The analysis is performed at a flow rate of 1.0 ml/min, with a 20-minute run time and 20 µL injection volume.
