Methods, application insights, and new column updates
Separation of Related Substances of Fluorouracil_USP Method
Application ID :
AP_1170
Analytes
- Unknown Impurity-1, Fluorouracil Related Compound-A, Unknown Impurity-2, Fluorouracil Related Compound-B, Uracil, Fluorouracil, Unknown Impurity-3, Fluorouracil Related Compound-E
Conditions
Column : Zodiac-Phenyl Column
Column Size : 250mmx4.6mm, 4µm
Mobile Phase :
M.P.: 0.77g/L of Ammonium acetate in water & adjust with formic acid to a pH of 5.60-5.65
Flow Rate : 10ml/min
Description
To separate related substances of Fluorouracil using a Zodiac Phenyl column in high-performance liquid chromatography (HPLC) as outlined in the USP method. The USP method for Fluorouracil typically employs a mobile phase that might include a combination of water, acetonitrile, and a buffer. Prepare a standard solution of Fluorouracil with a known concentration. This solution will be used to create a calibration curve and to identify peaks. Equilibrate the Zodiac Phenyl column with the mobile phase before use. Ensure the system is stabilized.
