Separation of Related Substances of Fluorouracil_USP Method

To separate related substances of Fluorouracil using a Zodiac Phenyl column in high-performance liquid chromatography (HPLC) as outlined in the USP method. The USP method for Fluorouracil typically employs a mobile phase that might include a combination of water, acetonitrile, and a buffer. Prepare a standard solution of Fluorouracil with a known concentration. This solution will be used to create a calibration curve and to identify peaks. Equilibrate the Zodiac Phenyl column with the mobile phase before use. Ensure the system is stabilized.