Methods, application insights, and new column updates
English | Global
Menu
Categories
We have imported template successfully. To setup it in the correct way please, save this page, refresh and select it in dropdown.
Separation of Related Substances of Fluorouracil_USP Method
Application ID :
AP_1170
Analytes
- Unknown Impurity-1, Fluorouracil Related Compound-A, Unknown Impurity-2, Fluorouracil Related Compound-B, Uracil, Fluorouracil, Unknown Impurity-3, Fluorouracil Related Compound-E
Conditions
Column : Zodiac-Phenyl Column
Column Size : 250mmx4.6mm, 4µm
Mobile Phase :
M.P.: 0.77g/L of Ammonium acetate in water & adjust with formic acid to a pH of 5.60-5.65
Flow Rate : 10ml/min
Buffer Details : To separate related substances of Fluorouracil using a Zodiac Phenyl column in high-performance liquid chromatography (HPLC) as outlined in the USP method. The USP method for Fluorouracil typically employs a mobile phase that might include a combination of water, acetonitrile, and a buffer. Prepare a standard solution of Fluorouracil with a known concentration. This solution will be used to create a calibration curve and to identify peaks. Equilibrate the Zodiac Phenyl column with the mobile phase before use. Ensure the system is stabilized.
Detector : UV 254 nm
Column Temperature : 25°C
Sample Temperature : 25°C
Injection Volume : 10 μι
Diluent/Sample Solvent : Water
Description
To separate related substances of Fluorouracil using a Zodiac Phenyl column in high-performance liquid chromatography (HPLC) as outlined in the USP method. The USP method for Fluorouracil typically employs a mobile phase that might include a combination of water, acetonitrile, and a buffer. Prepare a standard solution of Fluorouracil with a known concentration. This solution will be used to create a calibration curve and to identify peaks. Equilibrate the Zodiac Phenyl column with the mobile phase before use. Ensure the system is stabilized.
