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Separation of Ranolazine
Application ID :
AP_1161
Analytes
- Ranolazine
Conditions
Column : Zodiac 120 C8 AQ HPLC Column
Column Size : 250mm * 4.6mm, 5mu*m
Mobile Phase :
Buffer: weigh 1.6g of NaH2PO4.2H2O/1L water & adjust pH 3.0 with H3PO4
Buffer: Acetonitrile (80:20)
Acetonitrile: Water (70:30)
Flow Rate : 1.0 ml/min
Buffer Details : Separation of ranolazine using a C8 aqueous column involves using a column packed with octylsilane-bonded silica, which retains moderately hydrophobic compounds. The C8 column provides a less hydrophobic environment than C18 columns, aiding in the separation of ranolazine from other components. The aqueous mobile phase, combined with organic solvents, interacts differentially with ranolazine, leading to distinct retention times and better resolution of the compound. This setup improves the accuracy and efficiency of analyzing ranolazine in various samples.
Detector : UV 220 nm
Column Temperature : 25°C
Sample Temperature : 25°C
Injection Volume : 10μί
Diluent/Sample Solvent : Mobile phase-A
Description
Separation of ranolazine using a C8 aqueous column involves using a column packed with octylsilane-bonded silica, which retains moderately hydrophobic compounds. The C8 column provides a less hydrophobic environment than C18 columns, aiding in the separation of ranolazine from other components. The aqueous mobile phase, combined with organic solvents, interacts differentially with ranolazine, leading to distinct retention times and better resolution of the compound. This setup improves the accuracy and efficiency of analyzing ranolazine in various samples.
