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Column : Presto 120 C8 AQ Column
Column Size : 150mm x 4.6mm,2.7µm
Mobile Phase :
n-propyl alcohol and buffer (27:73). (Note: The ratio of in Buffer be varied between 26:74 and 29:71 to meet system suitability requirements.)
Solution A : 280g/L of potassium hydroxide in water.
Flow Rate : 0.5 mL/min
Buffer Details : 2.9 g/L of in Adjust with solution A to a pH of 2.5 to 1.0L of this solution add 5.9 g of octanesulfnnic acid sodium salt monohydrate
Detector : UV 215 nm
Elution Mode : Isocratic
Run Time : 25 min
Column Temperature : 40°C
Injection Volume : 10 µL
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