Methods, application insights, and new column updates
English | Global
Menu
Categories
We have imported template successfully. To setup it in the correct way please, save this page, refresh and select it in dropdown.
USP Content of 4-Aminophenol in Acetaminophen by HPLC
Application ID :
AN-1229/2
Analytes
- 4-Aminophenol, 2.Acetaminophen
Conditions
Column : Zodiac HST OG Column
Column Size : 250mm x 4.6mm,5µm
Mobile Phase :
Solution A : 10 mM phosphate buffer prepared : Add 0.60 g of mono basic potassium phosphate and 0.82g of anhydrous dibasic sodium phosphate to a 1L volumetric flask. Dissolve and dilute with water to to a pH of 7.0.
Solution B : Water
Solution C : Acetonitrile
Flow Rate : 1.0 mL/min
Buffer Details : 40 g/L of sodium citrate dihydrate and 1.5 g/L of anhydrous citric acid in water
Detector : 300 nm
Elution Mode : Gradient Program
Run Time : 10 min
Column Temperature : 30°C
Injection Volume : 10 µL
Description
4-Aminophenol (also known as p-aminophenol) is the primary degradation product and impurity (impurity K) in acetaminophen (paracetamol) containing drug products, formed via the hydrolysis of the active pharmaceutical ingredient.The method provides a clear seperation of chromatographic peak of 4-Amino phenol and Acetaminophen eluting at 3.578 and 4.586 minutes respectively.
