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IP Assay for Gabapentin by HPLC

Application ID : AN-1227

Analytes

  1. Gabapentin
Conditions

Column : Presto 120 C18 Column

Column Size : 150mm x 4.6mm,2.7µm

Mobile Phase :
a mixture of 24 volumes of acetonitrile and 76 volumes of buffer solution prepared by dissolving 0.58g of mmonium dihydrogen phosphate and 1.86g of sodium perchlorate in 950 ml of water and adjusted to pH 1.8 with perchloric acid and diluted to 1000 ml with water

Flow Rate : 0.5 mL/min

Detector : UV 215 nm

Elution Mode : Isocratic

Run Time : 10 min

Column Temperature : 40°C

Injection Volume : 20 µL

Description
Gabapentin is an anticonvulsant (also known as an anti-seizure medication) and a gabapentinoid nerve-stabilizing drug.The method provides a clear seperation of chromatographic peak of Gabapentin eluting at ,3.910 minutes