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Column : Presto 120 C18 Column
Column Size : 150mm x 4.6mm,2.7µm
Mobile Phase :
a mixture of 24 volumes of acetonitrile and 76 volumes of buffer solution prepared by dissolving 0.58g of mmonium dihydrogen phosphate and 1.86g of sodium perchlorate in 950 ml of water and adjusted to pH 1.8 with perchloric acid and diluted to 1000 ml with water
Flow Rate : 0.5 mL/min
Detector : UV 215 nm
Elution Mode : Isocratic
Run Time : 10 min
Column Temperature : 40°C
Injection Volume : 20 µL
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