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Assay Of L-Arginine Using USP_32 Method

Application ID : 28

Analytes

  1. L-Arginine
Conditions

Column : Zodiac C8(1) Column

Column Size : 50mm * 4.6mm, 3mu*m

Mobile Phase :
Buffer: Weigh about 2.0g of KH2PO4/1L Water & adjust pH 6.0 with NaOH Solution, M.P.A: Methanol: Buffer (10:90), M.P.B: Buffer: Methanol (10:90)

Flow Rate : 1.2 ml/min

Detection : UV 224 nm

Description
The Zodiac C8(1) column is a reversed-phase chromatography stationary phase with C8 (octyl) chains bonded to silica, providing moderate hydrophobicity for diverse separations. It offers good resolution and selectivity for small to medium-sized molecules in various analytical applications.