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Column : Zodiac C4 HPLC Column
Column Size : 150x4.6mm, 3µm
Mobile Phase :
0.41g EDTA & 1.7g K2HPO4, 1.7g [CH3(CH2)3]4N.H2PO4 in 900ml H2O, adjust pH 7.5 with 1M NaOH & dilute to
1000ml millig water
Buffer: Acetonitrile (900:100)
Flow Rate : 1.0 ml/min
Detection : UV 263 nm
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