Zodiac HST RP

• Streamlined Workflow: Eliminates the need for solid-phase extraction, liquid-liquid extraction, centrifugation, or filtration, reducing cost and effort while improving accuracy.
• Plasma Component Exclusion: Effectively excludes high-molecular-weight proteins and peptides to protect the column, ensuring consistent performance and preventing irreversible adsorption.
• Enhanced Retention: Separates small negatively charged molecules via anion-exchange or reverse-phase modes, and hydrophobic molecules through regular reverse-phase chromatography.
• High Selectivity and Recovery: Delivers sharp peaks for small molecules, while proteins and peptides elute in the void time, avoiding interference with analyte detection.
• Versatile Compatibility: Works seamlessly with LC-MS, preparative chromatography, and low UV detection, using mobile phases such as MeCN-H₂O with formic acid for pH adjustment.
• Versatile Compatibility: Works seamlessly with LC-MS, preparative chromatography, and low UV detection, using mobile phases such as MeCN-H₂O with formic acid for pH adjustment.

Innovative Stationary Phase Design:

The Zodiac HST RP column employs a unique stationary phase comprising dual functional groups:

• Anion-Exchange Group: Enables separation of negatively charged molecules.
• Long Hydrophobic Alkyl Chain: Retains hydrophobic molecules through reverse-phase mechanisms.

At pH ~3.0, most proteins in plasma become positively charged and are excluded from interaction with the stationary phase. This ensures clean separations and protects the column from degradation.

Simplified Protein Management:

Proteins and peptides elute as a sharp peak in void or pre-void time, which can be diverted to waste using a bypass valve during the first 40 seconds for a 50 mm column (time may vary for longer columns).