Zodiac C8- I3 Column

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Zodiac C8- i3 Column

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Zodiac C8-i3column, An excellent resolution for critical separations, faster analysis compared to C18 increased selectivity, exceptional reproducibility. Zodiac C8-i3 is an admirable choice for separations that need marginally more polar phase than C18. Zodiac C8-i3 column provides additional resolution to polar phase. Zodiac C8-i3 column is less hydrophobic column with high surface area, optimal carbon load and unique bonded silica packing.

This column is ideal choice when analytical laboratories need extra selectivity and more speed than the C18 column. The superior resolution of Zodiac C8-i3 yields out and out separation first time, speeding up method development and validation. These columns are highly stable and produce ever reliable results with an extended column life.

The column comes with protective end-capping for a better peak without tailing and high durability of column. Faster, sharp and reproducible responses are definitely possible with Zodiac C8-i3 columns. Column packing sustains any pH range between 1.5 and 8 for longer periods due to the highly innovative bonding technology.

Technical Details

Silica Type : Spherical

Silica Purity : 99.999%

Carbon Load : 12 %

Surface Area : 340 m2/gram

Pore Size : 100A

PH Range : 2 to 8

Particle Size : 5um and 10um

Details will be updated soon……!

Analytical Columns

Part numberPhaseparticle size (µm)length (mm)ID (mm)
ZLS.C8i3.46.300.0510Zodiac C8-i353004.6
ZLS.C8i3.40.300.0510Zodiac C8-i353004
ZLS.C8i3.46.250.0510Zodiac C8-i352504.6
ZLS.C8i3.40.250.0510Zodiac C8-i352504
ZLS.C8i3.30.250.0510Zodiac C8-i352503
ZLS.C8i3.20.250.0510Zodiac C8-i352502
ZLS.C8i3.10.250.0510Zodiac C8-i352501
ZLS.C8i3.46.200.0510Zodiac C8-i352004.6
ZLS.C8i3.40.200.0510Zodiac C8-i352004
ZLS.C8i3.30.200.0510Zodiac C8-i352003
ZLS.C8i3.20.200.0510Zodiac C8-i352002
ZLS.C8i3.10.200.0510Zodiac C8-i352001
ZLS.C8i3.46.150.0510Zodiac C8-i351504.6
ZLS.C8i3.40.150.0510Zodiac C8-i351504
ZLS.C8i3.30.150.0510Zodiac C8-i351503
ZLS.C8i3.20.150.0510Zodiac C8-i351502
ZLS.C8i3.10.150.0510Zodiac C8-i351501
ZLS.C8i3.46.125.0510Zodiac C8-i351254.6
ZLS.C8i3.40.125.0510Zodiac C8-i351254
ZLS.C8i3.30.125.0510Zodiac C8-i351253
ZLS.C8i3.20.125.0510Zodiac C8-i351252
ZLS.C8i3.10.125.0510Zodiac C8-i351251
ZLS.C8i3.46.100.0510Zodiac C8-i351004.6
ZLS.C8i3.40.100.0510Zodiac C8-i351004
ZLS.C8i3.30.100.0510Zodiac C8-i351003
ZLS.C8i3.20.100.0510Zodiac C8-i351002
ZLS.C8i3.10.100.0510Zodiac C8-i351001
ZLS.C8i3.46.75.0510Zodiac C8-i35754.6
ZLS.C8i3.40.75.0510Zodiac C8-i35754
ZLS.C8i3.30.75.0510Zodiac C8-i35753
ZLS.C8i3.20.75.0510Zodiac C8-i35752
ZLS.C8i3.10.75.0510Zodiac C8-i35751
ZLS.C8i3.46.50.0510Zodiac C8-i35504.6
ZLS.C8i3.40.50.0510Zodiac C8-i35504
ZLS.C8i3.30.50.0510Zodiac C8-i35503
ZLS.C8i3.20.50.0510Zodiac C8-i35502
ZLS.C8i3.10.50.0510Zodiac C8-i35501
ZLS.C8i3.46.33.0510Zodiac C8-i35334.6
ZLS.C8i3.40.33.0510Zodiac C8-i35334
ZLS.C8i3.30.33.0510Zodiac C8-i35333
ZLS.C8i3.20.33.0510Zodiac C8-i35332
ZLS.C8i3.10.33.0510Zodiac C8-i35331
ZLS.C8i3.46.30.0510Zodiac C8-i35304.6
ZLS.C8i3.40.30.0510Zodiac C8-i35304
ZLS.C8i3.30.30.0510Zodiac C8-i35303
ZLS.C8i3.20.30.0510Zodiac C8-i35302
ZLS.C8i3.10.30.0510Zodiac C8-i35301
ZLS.C8i3.20.20.0510Zodiac C8-i35202
ZLS.C8i3.46.300.0310Zodiac C8-i333004.6
ZLS.C8i3.40.300.0310Zodiac C8-i333004
ZLS.C8i3.46.250.0310Zodiac C8-i332504.6
ZLS.C8i3.40.250.0310Zodiac C8-i332504
ZLS.C8i3.30.250.0310Zodiac C8-i332503
ZLS.C8i3.20.250.0310Zodiac C8-i332502
ZLS.C8i3.10.250.0310Zodiac C8-i332501
ZLS.C8i3.46.200.0310Zodiac C8-i332004.6
ZLS.C8i3.40.200.0310Zodiac C8-i332004
ZLS.C8i3.30.200.0310Zodiac C8-i332003
ZLS.C8i3.20.200.0310Zodiac C8-i332002
ZLS.C8i3.10.200.0310Zodiac C8-i332001
ZLS.C8i3.46.150.0310Zodiac C8-i331504.6
ZLS.C8i3.40.150.0310Zodiac C8-i331504
ZLS.C8i3.30.150.0310Zodiac C8-i331503
ZLS.C8i3.20.150.0310Zodiac C8-i331502
ZLS.C8i3.10.150.0310Zodiac C8-i331501
ZLS.C8i3.46.125.0310Zodiac C8-i331254.6
ZLS.C8i3.40.125.0310Zodiac C8-i331254
ZLS.C8i3.30.125.0310Zodiac C8-i331253
ZLS.C8i3.20.125.0310Zodiac C8-i331252
ZLS.C8i3.10.125.0310Zodiac C8-i331251
ZLS.C8i3.46.100.0310Zodiac C8-i331004.6
ZLS.C8i3.40.100.0310Zodiac C8-i331004
ZLS.C8i3.30.100.0310Zodiac C8-i331003
ZLS.C8i3.20.100.0310Zodiac C8-i331002
ZLS.C8i3.10.100.0310Zodiac C8-i331001
ZLS.C8i3.46.75.0310Zodiac C8-i33754.6
ZLS.C8i3.40.75.0310Zodiac C8-i33754
ZLS.C8i3.30.75.0310Zodiac C8-i33753
ZLS.C8i3.20.75.0310Zodiac C8-i33752
ZLS.C8i3.10.75.0310Zodiac C8-i33751
ZLS.C8i3.46.50.0310Zodiac C8-i33504.6
ZLS.C8i3.40.50.0310Zodiac C8-i33504
ZLS.C8i3.30.50.0310Zodiac C8-i33503
ZLS.C8i3.20.50.0310Zodiac C8-i33502
ZLS.C8i3.10.50.0310Zodiac C8-i33501
ZLS.C8i3.46.33.0310Zodiac C8-i33334.6
ZLS.C8i3.40.33.0310Zodiac C8-i33334
ZLS.C8i3.30.33.0310Zodiac C8-i33333
ZLS.C8i3.20.33.0310Zodiac C8-i33332
ZLS.C8i3.10.33.0310Zodiac C8-i33331
ZLS.C8i3.46.30.0310Zodiac C8-i33304.6
ZLS.C8i3.40.30.0310Zodiac C8-i33304
ZLS.C8i3.30.30.0310Zodiac C8-i33303
ZLS.C8i3.20.30.0310Zodiac C8-i33302
ZLS.C8i3.10.30.0310Zodiac C8-i33301
ZLS.C8i3.20.20.0310Zodiac C8-i33202
ZLS.C8i3.46.300.1010Zodiac C8-i3103004.6
ZLS.C8i3.40.300.1010Zodiac C8-i3103004
ZLS.C8i3.46.250.1010Zodiac C8-i3102504.6
ZLS.C8i3.40.250.1010Zodiac C8-i3102504
ZLS.C8i3.30.250.1010Zodiac C8-i3102503
ZLS.C8i3.20.250.1010Zodiac C8-i3102502
ZLS.C8i3.10.250.1010Zodiac C8-i3102501
ZLS.C8i3.46.200.1010Zodiac C8-i3102004.6
ZLS.C8i3.40.200.1010Zodiac C8-i3102004
ZLS.C8i3.30.200.1010Zodiac C8-i3102003
ZLS.C8i3.20.200.1010Zodiac C8-i3102002
ZLS.C8i3.10.200.1010Zodiac C8-i3102001
ZLS.C8i3.46.150.1010Zodiac C8-i3101504.6
ZLS.C8i3.40.150.1010Zodiac C8-i3101504
ZLS.C8i3.30.150.1010Zodiac C8-i3101503
ZLS.C8i3.20.150.1010Zodiac C8-i3101502
ZLS.C8i3.10.150.1010Zodiac C8-i3101501
ZLS.C8i3.46.125.1010Zodiac C8-i3101254.6
ZLS.C8i3.40.125.1010Zodiac C8-i3101254
ZLS.C8i3.30.125.1010Zodiac C8-i3101253
ZLS.C8i3.20.125.1010Zodiac C8-i3101252
ZLS.C8i3.10.125.1010Zodiac C8-i3101251
ZLS.C8i3.46.100.1010Zodiac C8-i3101004.6
ZLS.C8i3.40.100.1010Zodiac C8-i3101004
ZLS.C8i3.30.100.1010Zodiac C8-i3101003
ZLS.C8i3.20.100.1010Zodiac C8-i3101002
ZLS.C8i3.10.100.1010Zodiac C8-i3101001
ZLS.C8i3.46.75.1010Zodiac C8-i310754.6
ZLS.C8i3.40.75.1010Zodiac C8-i310754
ZLS.C8i3.30.75.1010Zodiac C8-i310753
ZLS.C8i3.20.75.1010Zodiac C8-i310752
ZLS.C8i3.10.75.1010Zodiac C8-i310751
ZLS.C8i3.46.50.1010Zodiac C8-i310504.6
ZLS.C8i3.40.50.1010Zodiac C8-i310504
ZLS.C8i3.30.50.1010Zodiac C8-i310503
ZLS.C8i3.20.50.1010Zodiac C8-i310502
ZLS.C8i3.10.50.1010Zodiac C8-i310501
ZLS.C8i3.46.33.1010Zodiac C8-i310334.6
ZLS.C8i3.40.33.1010Zodiac C8-i310334
ZLS.C8i3.30.33.1010Zodiac C8-i310333
ZLS.C8i3.20.33.1010Zodiac C8-i310332
ZLS.C8i3.10.33.1010Zodiac C8-i310331
ZLS.C8i3.46.30.1010Zodiac C8-i310304.6
ZLS.C8i3.40.30.1010Zodiac C8-i310304
ZLS.C8i3.30.30.1010Zodiac C8-i310303
ZLS.C8i3.20.30.1010Zodiac C8-i310302
ZLS.C8i3.10.30.1010Zodiac C8-i310301
ZLS.C8i3.20.20.1010Zodiac C8-i310202

Zodiac Preparative Columns

Part numberPhaseparticle size (µm)length (mm)ID (mm)
ZLS.C8i3P.100.50.1010Zodiac C8-i3 Prep105010
ZLS.C8i3P.200.50.1010Zodiac C8-i3 Prep105020
ZLS.C8i3P.300.50.1010Zodiac C8-i3 Prep105030
ZLS.C8i3P.400.50.1010Zodiac C8-i3 Prep105040
ZLS.C8i3P.500.50.1010Zodiac C8-i3 Prep105050
ZLS.C8i3P.100.100.1010Zodiac C8-i3 Prep1010010
ZLS.C8i3P.200.100.1010Zodiac C8-i3 Prep1010020
ZLS.C8i3P.300.100.1010Zodiac C8-i3 Prep1010030
ZLS.C8i3P.400.100.1010Zodiac C8-i3 Prep1010040
ZLS.C8i3P.500.100.1010Zodiac C8-i3 Prep1010050
ZLS.C8i3P.100.150.1010Zodiac C8-i3 Prep1015010
ZLS.C8i3P.200.150.1010Zodiac C8-i3 Prep1015020
ZLS.C8i3P.300.150.1010Zodiac C8-i3 Prep1015030
ZLS.C8i3P.400.150.1010Zodiac C8-i3 Prep1015040
ZLS.C8i3P.500.150.1010Zodiac C8-i3 Prep1015050
ZLS.C8i3P.100.200.1010Zodiac C8-i3 Prep1020010
ZLS.C8i3P.200.200.1010Zodiac C8-i3 Prep1020020
ZLS.C8i3P.300.200.1010Zodiac C8-i3 Prep1020030
ZLS.C8i3P.400.200.1010Zodiac C8-i3 Prep1020040
ZLS.C8i3P.500.200.1010Zodiac C8-i3 Prep1020050
ZLS.C8i3P.100.250.1010Zodiac C8-i3 Prep1025010
ZLS.C8i3P.200.250.1010Zodiac C8-i3 Prep1025020
ZLS.C8i3P.300.250.1010Zodiac C8-i3 Prep1025030
ZLS.C8i3P.400.250.1010Zodiac C8-i3 Prep1025040
ZLS.C8i3P.500.250.1010Zodiac C8-i3 Prep1025050
ZLS.C8i3P.100.300.1010Zodiac C8-i3 Prep1030010
ZLS.C8i3P.200.300.1010Zodiac C8-i3 Prep1030020
ZLS.C8i3P.300.300.1010Zodiac C8-i3 Prep1030030
ZLS.C8i3P.400.300.1010Zodiac C8-i3 Prep1030040
ZLS.C8i3P.500.300.1010Zodiac C8-i3 Prep1030050
ZLS.C8i3P.100.500.1010Zodiac C8-i3 Prep1050010
ZLS.C8i3P.200.500.1010Zodiac C8-i3 Prep1050020
ZLS.C8i3P.300.500.1010Zodiac C8-i3 Prep1050030
ZLS.C8i3P.400.500.1010Zodiac C8-i3 Prep1050040
ZLS.C8i3P.500.500.1010Zodiac C8-i3 Prep1050050
ZLS.C8i3P.100.50.1510Zodiac C8-i3 Prep155010
ZLS.C8i3P.200.50.1510Zodiac C8-i3 Prep155020
ZLS.C8i3P.300.50.1510Zodiac C8-i3 Prep155030
ZLS.C8i3P.400.50.1510Zodiac C8-i3 Prep155040
ZLS.C8i3P.500.50.1510Zodiac C8-i3 Prep155050
ZLS.C8i3P.100.100.1510Zodiac C8-i3 Prep1510010
ZLS.C8i3P.200.100.1510Zodiac C8-i3 Prep1510020
ZLS.C8i3P.300.100.1510Zodiac C8-i3 Prep1510030
ZLS.C8i3P.400.100.1510Zodiac C8-i3 Prep1510040
ZLS.C8i3P.500.100.1510Zodiac C8-i3 Prep1510050
ZLS.C8i3P.100.150.1510Zodiac C8-i3 Prep1515010
ZLS.C8i3P.200.150.1510Zodiac C8-i3 Prep1515020
ZLS.C8i3P.300.150.1510Zodiac C8-i3 Prep1515030
ZLS.C8i3P.400.150.1510Zodiac C8-i3 Prep1515040
ZLS.C8i3P.500.150.1510Zodiac C8-i3 Prep1515050
ZLS.C8i3P.100.200.1510Zodiac C8-i3 Prep1520010
ZLS.C8i3P.200.200.1510Zodiac C8-i3 Prep1520020
ZLS.C8i3P.300.200.1510Zodiac C8-i3 Prep1520030
ZLS.C8i3P.400.200.1510Zodiac C8-i3 Prep1520040
ZLS.C8i3P.500.200.1510Zodiac C8-i3 Prep1520050
ZLS.C8i3P.100.250.1510Zodiac C8-i3 Prep1525010
ZLS.C8i3P.200.250.1510Zodiac C8-i3 Prep1525020
ZLS.C8i3P.300.250.1510Zodiac C8-i3 Prep1525030
ZLS.C8i3P.400.250.1510Zodiac C8-i3 Prep1525040
ZLS.C8i3P.500.250.1510Zodiac C8-i3 Prep1525050
ZLS.C8i3P.100.300.1510Zodiac C8-i3 Prep1530010
ZLS.C8i3P.200.300.1510Zodiac C8-i3 Prep1530020
ZLS.C8i3P.300.300.1510Zodiac C8-i3 Prep1530030
ZLS.C8i3P.400.300.1510Zodiac C8-i3 Prep1530040
ZLS.C8i3P.500.300.1510Zodiac C8-i3 Prep1530050
ZLS.C8i3P.100.500.1510Zodiac C8-i3 Prep1550010
ZLS.C8i3P.200.500.1510Zodiac C8-i3 Prep1550020
ZLS.C8i3P.300.500.1510Zodiac C8-i3 Prep1550030
ZLS.C8i3P.400.500.1510Zodiac C8-i3 Prep1550040
ZLS.C8i3P.500.500.1510Zodiac C8-i3 Prep1550050
ZLS.C8i3P.100.50.2010Zodiac C8-i3 Prep205010
ZLS.C8i3P.200.50.2010Zodiac C8-i3 Prep205020
ZLS.C8i3P.300.50.2010Zodiac C8-i3 Prep205030
ZLS.C8i3P.400.50.2010Zodiac C8-i3 Prep205040
ZLS.C8i3P.500.50.2010Zodiac C8-i3 Prep205050
ZLS.C8i3P.100.100.2010Zodiac C8-i3 Prep2010010
ZLS.C8i3P.200.100.2010Zodiac C8-i3 Prep2010020
ZLS.C8i3P.300.100.2010Zodiac C8-i3 Prep2010030
ZLS.C8i3P.400.100.2010Zodiac C8-i3 Prep2010040
ZLS.C8i3P.500.100.2010Zodiac C8-i3 Prep2010050
ZLS.C8i3P.100.150.2010Zodiac C8-i3 Prep2015010
ZLS.C8i3P.200.150.2010Zodiac C8-i3 Prep2015020
ZLS.C8i3P.300.150.2010Zodiac C8-i3 Prep2015030
ZLS.C8i3P.400.150.2010Zodiac C8-i3 Prep2015040
ZLS.C8i3P.500.150.2010Zodiac C8-i3 Prep2015050
ZLS.C8i3P.100.200.2010Zodiac C8-i3 Prep2020010
ZLS.C8i3P.200.200.2010Zodiac C8-i3 Prep2020020
ZLS.C8i3P.300.200.2010Zodiac C8-i3 Prep2020030
ZLS.C8i3P.400.200.2010Zodiac C8-i3 Prep2020040
ZLS.C8i3P.500.200.2010Zodiac C8-i3 Prep2020050
ZLS.C8i3P.100.250.2010Zodiac C8-i3 Prep2025010
ZLS.C8i3P.200.250.2010Zodiac C8-i3 Prep2025020
ZLS.C8i3P.300.250.2010Zodiac C8-i3 Prep2025030
ZLS.C8i3P.400.250.2010Zodiac C8-i3 Prep2025040
ZLS.C8i3P.500.250.2010Zodiac C8-i3 Prep2025050
ZLS.C8i3P.100.300.2010Zodiac C8-i3 Prep2030010
ZLS.C8i3P.200.300.2010Zodiac C8-i3 Prep2030020
ZLS.C8i3P.300.300.2010Zodiac C8-i3 Prep2030030
ZLS.C8i3P.400.300.2010Zodiac C8-i3 Prep2030040
ZLS.C8i3P.500.300.2010Zodiac C8-i3 Prep2030050
ZLS.C8i3P.100.500.2010Zodiac C8-i3 Prep2050010
ZLS.C8i3P.200.500.2010Zodiac C8-i3 Prep2050020
ZLS.C8i3P.300.500.2010Zodiac C8-i3 Prep2050030
ZLS.C8i3P.400.500.2010Zodiac C8-i3 Prep2050040
ZLS.C8i3P.500.500.2010Zodiac C8-i3 Prep2050050
ZLS.C8i3P.100.50.3010Zodiac C8-i3 Prep305010
ZLS.C8i3P.200.50.3010Zodiac C8-i3 Prep305020
ZLS.C8i3P.300.50.3010Zodiac C8-i3 Prep305030
ZLS.C8i3P.400.50.3010Zodiac C8-i3 Prep305040
ZLS.C8i3P.500.50.3010Zodiac C8-i3 Prep305050
ZLS.C8i3P.100.100.3010Zodiac C8-i3 Prep3010010
ZLS.C8i3P.200.100.3010Zodiac C8-i3 Prep3010020
ZLS.C8i3P.300.100.3010Zodiac C8-i3 Prep3010030
ZLS.C8i3P.400.100.3010Zodiac C8-i3 Prep3010040
ZLS.C8i3P.500.100.3010Zodiac C8-i3 Prep3010050
ZLS.C8i3P.100.150.3010Zodiac C8-i3 Prep3015010
ZLS.C8i3P.200.150.3010Zodiac C8-i3 Prep3015020
ZLS.C8i3P.300.150.3010Zodiac C8-i3 Prep3015030
ZLS.C8i3P.400.150.3010Zodiac C8-i3 Prep3015040
ZLS.C8i3P.500.150.3010Zodiac C8-i3 Prep3015050
ZLS.C8i3P.100.200.3010Zodiac C8-i3 Prep3020010
ZLS.C8i3P.200.200.3010Zodiac C8-i3 Prep3020020
ZLS.C8i3P.300.200.3010Zodiac C8-i3 Prep3020030
ZLS.C8i3P.400.200.3010Zodiac C8-i3 Prep3020040
ZLS.C8i3P.500.200.3010Zodiac C8-i3 Prep3020050
ZLS.C8i3P.100.250.3010Zodiac C8-i3 Prep3025010
ZLS.C8i3P.200.250.3010Zodiac C8-i3 Prep3025020
ZLS.C8i3P.300.250.3010Zodiac C8-i3 Prep3025030
ZLS.C8i3P.400.250.3010Zodiac C8-i3 Prep3025040
ZLS.C8i3P.500.250.3010Zodiac C8-i3 Prep3025050
ZLS.C8i3P.100.300.3010Zodiac C8-i3 Prep3030010
ZLS.C8i3P.200.300.3010Zodiac C8-i3 Prep3030020
ZLS.C8i3P.300.300.3010Zodiac C8-i3 Prep3030030
ZLS.C8i3P.400.300.3010Zodiac C8-i3 Prep3030040
ZLS.C8i3P.500.300.3010Zodiac C8-i3 Prep3030050
ZLS.C8i3P.100.500.3010Zodiac C8-i3 Prep3050010
ZLS.C8i3P.200.500.3010Zodiac C8-i3 Prep3050020
ZLS.C8i3P.300.500.3010Zodiac C8-i3 Prep3050030
ZLS.C8i3P.400.500.3010Zodiac C8-i3 Prep3050040
ZLS.C8i3P.500.500.3010Zodiac C8-i3 Prep3050050

Zodiac Guard Columns

Part numberPhaseparticle size (µm)length (mm)ID (mm)
ZLS.C8i3.40.10.5GZodiac C8-i35104
ZLS.C8i3.46.30.5GZodiac C8-i35304.6
ZLS.C8i3.40.30.5GZodiac C8-i35304
ZLS.C8i3.46.33.5GZodiac C8-i35334.6
ZLS.C8i3.40.33.5GZodiac C8-i35334
ZLS.C8i3.46.10.3GZodiac C8-i33104.6
ZLS.C8i3.40.10.3GZodiac C8-i33104
ZLS.C8i3.46.30.3GZodiac C8-i33304.6
ZLS.C8i3.40.30.3GZodiac C8-i33304
ZLS.C8i3.46.33.3GZodiac C8-i33334.6
ZLS.C8i3.40.33.3GZodiac C8-i33334

General HPLC Column Care And Use Instructions
Column Storage and Column Care
General Guidelines
Do not store HPLC columns in buffers. A buffer may precipitate inside the column, resulting in plugged hits and packing material. A buffer may also encourage bacterial growth, which can plug both the column frit and packing material. This is more likely to occur with mobile phases which have close to or 100% buffer. Bacteria may also affect your analytes, and organic products from the dead bacteria may cause “ghost peaks” in chromatograms. Do not store HPLC columns in solvents that degrade easily — tetrahydrofuran (THF), triethylamine (TEA), and trifluoroacetic acid (TFA). Unstabilized THF can form peroxides which may degrade the column. TEA and TFA are more likely to become contaminated from the lab environment and should be refrigerated during storage. Contamination may change the chromatography or change the column. Be aware of the quality issues with these mobile phase components when you use them for your chromatography.

Term Storage

Reversed-phase columns can also be stored in 100% acetonitrile. If you are using a buffered mobile phase, first flush the column with the mobile phase minus the buffer, then with 100% acetonitrile. In addition, a 100% ACN flush prior to long term storage can help remove contaminants from the column.

Regeneration of RP packings

RP- packings are C18, C8, C4, C1, C30, CN or Phenyl stationary phases
• Flush the column with 20? column volumes Water
• Flush the column with 20? column volumes Acetonitrile
• Flush the column with 5? column volumes Isopropanol
• Flush the column with 20? column volumes Heptane
• Flush the column with 5? column volumes Isopropanol
• Flush the column with 20 column volumes Acetonitrile

Regeneration of NP (Normal Phase) packings
NP-packings are Silica, Diol, Nitro and Amino stationary phases
Flush the column with 20 column volumes Heptane
Flush the column with 5 column volumes Isopropanol
Flush the column with 20 column volumes Acetonitrile
Flush the column with 20 column volumes Water
Flush the column with 20 column volumes Acetonitrile
Flush the column with 5 column volumes Isopropanol
Flush the column with 20 column volumes Heptane
Column ID Ideal Flow rate
2 0.2
3 0.5
4 1.0
4.6 1.0
8 3.0
10 5.0
20 20.0
32 48.0
50 120.0

Regeneration of Ion-Exchange packings

Anion- and Cation-Exchangers (WCX, SCX, WAX and SAX) are Ion-Exchange Packings
• Wash with 20 column volumes with the eluent, double the buffer concentration
• Wash with 20 column volumes Water (to elute out salts)
• Wash with 20 column volumes Acetonitrile (to elute hydrophilic contaminants)
• Wash with 5 column vol. Isopropanol (to elute mildly hydrophobic contaminants)
• Wash with 20 column volumes Heptane or Hexane or Methylenchloride (to elute strongly hydrophobic contaminants)
• Wash with 5 column volumes Isopropanol
• Wash with 20 column volumes Acetonitrile
• Wash with 20 column volumes Water
• Return the column to its original Ion-Exchange condition

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