Retention of Polar Compounds

This application demonstrates the effective separation of a mixture containing highly polar and hydrophobic compounds using the Zodiac HST OG mixed-mode column. Under gradient elution conditions, the column successfully retains and resolves compounds ranging from small polar amino acids to non-polar hydrocarbons like toluene.

In contrast, under nearly identical gradient conditions, a Leading C8 brand reversed-phase column failed to retain the highly polar components (e.g., amino acids) without the addition of ion-pairing agents. This highlights the Zodiac HST OG column’s ability to separate a broad polarity range of compounds based on a combination of hydrophobic and hydrogen-bonding interactions.

This method is particularly useful for complex sample matrices containing both polar and non-polar analytes and is fully compatible with UV, ELSD, CAD, and LC/MS detection methods.