HPLC Separation of Toluidine Isomers in Cation-Exchange Mode

Isomers of toluidine were successfully retained and separated in cation-exchange mode using the Zodiac HST S2 column. This column supports HILIC, cation-exchange, and anion-exclusion mechanisms, making it versatile for separating polar compounds. The method is fully compatible with UV, ELSD, and LC/MS detection, and is suitable for routine analysis of isomeric and polar molecules.