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Assay of Bilastin Stage-1 by HPLC
Application ID :
AN-1224
Analytes
- KSM-1, 2.Bilastine stage-1
Conditions
Column : Presto 120 C18 Column
Column Size : 150mm x 4.6mm,2.7µm
Mobile Phase :
Mix buffer and acetonitrile in the ratio of 1:1 %v/v.
Flow Rate : 0.5 mL/min
Buffer Details : Dissolve about 3.4g of potassium dihydrogen phosphate in 500 ml of water and mix. Adjust pH of this solution 3.5±0.05 with orthophosphoric acid.
Detector : UV 215 nm
Elution Mode : Gradient program
Run Time : 15 min
Column Temperature : 25°C
Injection Volume : 20 µL
Description
Bilastine is a second-generation, non-sedating, selective histamine H₁ receptor antagonist (antihistamine).This HPLC application demonstrates the separation of ksm-1 and bilastin using a Zodiac Carb column. The method provides a clear chromatographic peaks ksm-1 and bilastine stage-I eluting at , 6.042 and 6.328 respectively,
