Analysis of Methyl cholate, Cholic acid, Deoxycholic acid, Chenodeoxycholic acid on Zodiac HST B

Bile acids, derived from cholesterol, are amphipathic molecules critical for fat digestion and absorption. Synthesized in the liver and released into the small intestine, they emulsify dietary fats and undergo enterohepatic circulation.

• Cholic Acid: A primary bile acid synthesized in the liver, aiding in fat emulsification.
• Deoxycholic Acid: A secondary bile acid formed by bacterial conversion of cholic acid in the colon, supporting fat digestion.
• Chenodeoxycholic Acid: A primary bile acid synthesized in the liver, similar in function to cholic acid.
• Methyl Cholate: A methylated derivative of cholic acid, contributing to lipid solubilization.

Bile acids can be efficiently retained, separated, and analyzed using the Zodiac HST B mixed-mode column. This isocratic method uses a mobile phase of water, acetonitrile (MeCN), and sulfuric acid, with UV detection at 192 nm. This approach ensures reliable separation and analysis of bile acids.